J Proteome Res. 2007 Nov;6(11):4433-9. Epub 2007 Oct 10.

Characterization of amyloid beta peptides in cerebrospinal fluid by an automated immunoprecipitation procedure followed by mass spectrometry.

Portelius E, Tran AJ, Andreasson U, Persson R, Brinkmalm G, Zetterberg H, Blennow K, Westman-Brinkmalm A.

Source

Clinical Neurochemistry Laboratory, Department of Neuroscience and Physiology, University of Göteborg, Sahlgrenska University Hospital, S-431 80 Mölndal, Sweden. erik.portelius@neuro.gu.se

Abstract

Pathogenic events in Alzheimer's disease are believed to involve an imbalance between the production and clearance of the neurotoxic 42 amino acid form of the beta-amyloid peptide (Abeta1-42). Although much is known about the production of Abeta1-42, many questions remain about its degradation. Here, we describe an optimized automated immunoprecipitation mass spectrometry method that enables accurate and rapid monitoring of the major Abeta isoforms in cerebrospinal fluid. Furthermore, we describe a technique of antibody immobilization, minimizing background signals. The identities of these Abeta products were confirmed using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and nanoflow liquid chromatography and tandem mass spectrometry with a hybrid linear trap Fourier transform ion cyclotron resonance mass spectrometer. Finally, we report the finding of two novel Abeta peptides (Abeta2-17 and Abeta3-17).

PMID:: 17927230; [PubMed - indexed for MEDLINE]

LinkOut - more resources

Full Text Sources

Other Literature Sources

Labome Researcher Resource - ExactAntigen/Labome

Supplemental Content

Save items

Related citations in PubMed

Identification of novel APP/Abeta isoforms in human cerebrospinal fluid. [Neurodegener Dis. 2009]
Identification of novel APP/Abeta isoforms in human cerebrospinal fluid.
Portelius E, Brinkmalm G, Tran AJ, Zetterberg H, Westman-Brinkmalm A, Blennow K. Neurodegener Dis. 2009; 6(3):87-94. Epub 2009 Feb 20.
An Alzheimer's disease-specific beta-amyloid fragment signature in cerebrospinal fluid. [Neurosci Lett. 2006]
An Alzheimer's disease-specific beta-amyloid fragment signature in cerebrospinal fluid.
Portelius E, Zetterberg H, Andreasson U, Brinkmalm G, Andreasen N, Wallin A, Westman-Brinkmalm A, Blennow K. Neurosci Lett. 2006 Dec 6; 409(3):215-9. Epub 2006 Oct 17.
Determination of beta-amyloid peptide signatures in cerebrospinal fluid using immunoprecipitation-mass spectrometry. [J Proteome Res. 2006]
Determination of beta-amyloid peptide signatures in cerebrospinal fluid using immunoprecipitation-mass spectrometry.
Portelius E, Westman-Brinkmalm A, Zetterberg H, Blennow K. J Proteome Res. 2006 Apr; 5(4):1010-6.
Review Utility of mass spectrometry for proteome analysis: part I. Conceptual and experimental approaches. [Expert Rev Proteomics. 2008]
Review Utility of mass spectrometry for proteome analysis: part I. Conceptual and experimental approaches.
Ahmed FE. Expert Rev Proteomics. 2008 Dec; 5(6):841-64.
Review Offline and online liquid chromatography mass spectrometry in quantitative proteomics. [Comb Chem High Throughput Scre...]
Review Offline and online liquid chromatography mass spectrometry in quantitative proteomics.
Bereszczak JZ, Brancia FL. Comb Chem High Throughput Screen. 2009 Feb; 12(2):185-93.

See reviews... See all...

Cited by 11 PubMed Central articles

BACE1 inhibition induces a specific cerebrospinal fluid β-amyloid pattern that identifies drug effects in the central nervous system. [PLoS One. 2012]
BACE1 inhibition induces a specific cerebrospinal fluid β-amyloid pattern that identifies drug effects in the central nervous system.
Mattsson N, Rajendran L, Zetterberg H, Gustavsson M, Andreasson U, Olsson M, Brinkmalm G, Lundkvist J, Jacobson LH, Perrot L, et al. PLoS One. 2012; 7(2):e31084. Epub 2012 Feb 6.
Review A window into the heterogeneity of human cerebrospinal fluid Aβ peptides. [J Biomed Biotechnol. 2011]
Review A window into the heterogeneity of human cerebrospinal fluid Aβ peptides.
Ghidoni R, Paterlini A, Albertini V, Stoppani E, Binetti G, Fuxe K, Benussi L, Agnati LF. J Biomed Biotechnol. 2011; 2011:697036. Epub 2011 Aug 23.
Site-specific characterization of threonine, serine, and tyrosine glycosylations of amyloid precursor protein/amyloid beta-peptides in human cerebrospinal fluid. [Proc Natl Acad Sci U S A. 2011]
Site-specific characterization of threonine, serine, and tyrosine glycosylations of amyloid precursor protein/amyloid beta-peptides in human cerebrospinal fluid.
Halim A, Brinkmalm G, Rüetschi U, Westman-Brinkmalm A, Portelius E, Zetterberg H, Blennow K, Larson G, Nilsson J. Proc Natl Acad Sci U S A. 2011 Jul 19; 108(29):11848-53. Epub 2011 Jun 28.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Characterization of amyloid beta peptides in cerebrospinal fluid by an automated...
Characterization of amyloid beta peptides in cerebrospinal fluid by an automated immunoprecipitation procedure followed by mass spectrometry.
J Proteome Res. 2007 Nov ;6(11):4433-9. Epub 2007 Oct 10 .

PubMed
HPLC-PDA-MS/MS of anthocyanins and carotenoids from dovyalis and tamarillo fruit...
HPLC-PDA-MS/MS of anthocyanins and carotenoids from dovyalis and tamarillo fruits.
J Agric Food Chem. 2007 Oct 31 ;55(22):9135-41. Epub 2007 Oct 10 .

PubMed
Proteomic analysis of royal jelly from three strains of western honeybees (Apis ...
Proteomic analysis of royal jelly from three strains of western honeybees (Apis mellifera).
J Agric Food Chem. 2007 Oct 17 ;55(21):8411-22. Epub 2007 Oct 10 .

PubMed
Effect of medical honey on wounds colonised or infected with MRSA.
Effect of medical honey on wounds colonised or infected with MRSA.
J Wound Care. 2007 Sep ;16(8):325-8.

PubMed
The neurobiology of tobacco addiction.
The neurobiology of tobacco addiction.
Trends Pharmacol Sci. 1991 Dec ;12(12):467-73.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to:

Characterization of amyloid beta peptides in cerebrospinal fluid by an automated immunoprecipitation procedure followed by mass spectrometry.

Source

Abstract

Publication Types, MeSH Terms, Substances

Publication Types

MeSH Terms

Substances

LinkOut - more resources

Full Text Sources

Other Literature Sources

Supplemental Content

Save items

Related citations in PubMed

Cited by 11 PubMed Central articles

Related information

Recent activity

Simple NCBI Directory

Getting Started

Resources

Popular

Featured

NCBI Information