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Cell. 2007 Oct 5;131(1):146-59.

MiR-150 controls B cell differentiation by targeting the transcription factor c-Myb.

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  • 1The CBR Institute for Biomedical Research, Harvard Medical School, Boston, MA 02115, USA.

Abstract

MiR-150 is a microRNA (miRNA) specifically expressed in mature lymphocytes, but not their progenitors. A top predicted target of miR-150 is c-Myb, a transcription factor controlling multiple steps of lymphocyte development. Combining loss- and gain-of-function gene targeting approaches for miR-150 with conditional and partial ablation of c-Myb, we show that miR-150 indeed controls c-Myb expression in vivo in a dose-dependent manner over a narrow range of miRNA and c-Myb concentrations and that this dramatically affects lymphocyte development and response. Our results identify a key transcription factor as a critical target of a stage-specifically expressed miRNA in lymphocytes and suggest that this and perhaps other miRNAs have evolved to control the expression of just a few critical target proteins in particular cellular contexts.

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PMID:
17923094
[PubMed - indexed for MEDLINE]
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