Effect of phorbol ester on protein phosphorylation in the central nervous system of the leech Hirudo medicinalis: a two-dimensional electrophoretical analysis

Comp Biochem Physiol B. 1991;99(4):859-64. doi: 10.1016/0305-0491(91)90154-6.

Abstract

1. Proteins of different regions of the Hirudo medicinalis central nervous system have been analyzed by means of two-dimensional electrophoresis. 2. Subcellular distribution of phosphoproteins has been studied in leech segmental ganglia. 3. Phorbol 12,13-dibutyrate, a protein kinase C activator, stimulates the phosphorylation of a number of proteins whose isoelectric points and mol. wts are presented. 4. Putative roles for these phosphoproteins are discussed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Central Nervous System / metabolism
  • Electrophoresis, Gel, Two-Dimensional
  • Enzyme Activation
  • Ganglia / metabolism
  • Leeches
  • Phorbol 12,13-Dibutyrate / pharmacology*
  • Phosphorylation / drug effects
  • Protein Kinase C / metabolism*
  • Proteins / metabolism

Substances

  • Proteins
  • Phorbol 12,13-Dibutyrate
  • Protein Kinase C