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Nat Methods. 2007 Nov;4(11):919-21. Epub 2007 Sep 30.

An activity-independent selection system of thermostable protein variants.

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  • 1BiomĂ©thodes SA, Genavenir 8, 5 rue Henri Desbruères, 91030 Evry, France.


We describe an activity-independent method for the selection of thermostable mutants of any protein. It is based on a fusion construct comprising the protein of interest and a thermostable antibiotic resistance reporter, in such a way that thermostable mutants provide increased resistance in a thermophile. We isolated thermostable mutants of three human interferons and of two enzymes to demonstrate the applicability of the system.

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