Fast purification process optimization using mixed...[J Chromatogr A. 2008]

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1: J Chromatogr A. 2008 Jan 11;1177(2):226-33. Epub 2007 Sep 12.Links

Fast purification process optimization using mixed-mode chromatography sorbents in pre-packed mini-columns.

Brenac Brochier V, Schapman A, Santambien P, Britsch L.

Pall BioSepra, PALL France, 48 Avenue des Genottes, Cergy St. Christophe, France. virginie_brochier@europe.pall.com

Pre-packed MediaScout MiniChrom columns of 2.5, 5 and 10 mL were investigated for screening three mixed-mode chromatography sorbents (HEA, PPA and MEP HyperCel). Packing performance was of good quality and the three sorbents displayed higher capacity than traditional HIC sorbents in physiological-like conditions. Each sorbent offered a unique selectivity. Bovine beta-lactoglobulin was partially purified after loading milk whey directly on HEA HyperCel sorbent. The combination of small pre-packed columns and SELDI-MS appeared to be a valuable strategy for high-throughput screening of chromatography sorbents and for enabling rapid process development and optimization.

PMID: 17904149 [PubMed - indexed for MEDLINE]

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