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Methods. 2007 Oct;43(2):162-5.

Isolation of microRNA targets using biotinylated synthetic microRNAs.

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  • 1Biotech Research & Innovation Centre, Ole Maaløes Vej 5, 2200 Copenhagen, Denmark.

Abstract

MicroRNAs are small regulatory RNAs found in multicellular organisms where they post-transcriptionally regulate gene expression. In animals, microRNAs bind mRNAs via incomplete base pairings making the identification of microRNA targets inherently difficult. Here, we present a detailed method for experimental identification of microRNA targets based on affinity purification of tagged microRNAs associated with their targets.

PMID:
17889804
[PubMed - indexed for MEDLINE]
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