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1: Methods. 2007 Oct;43(2):140-5.Click here to read Links

Detection of microRNAs by Northern blot analyses using LNA probes.

Várallyay E, Burgyán J, Havelda Z.

Agricultural Biotechnology Center, Szent-Györgyi Albert ut 4, Gödöllö H-2100, Hungary.

MicroRNAs (miRNAs) are small, endogenous, non-coding RNA species, about 21 nucleotides in length, which modulate the expression of animal and plant target genes at the post-transcriptional level. It has been shown that miRNA based gene regulation plays a pivotal role in pathways involved in growth and development. Understanding miRNA mediated processes requires new technologies enabling efficient detection of small RNA species. Here we report the optimization of a miRNA Northern blot detection method based on LNA (locked nucleic acid)-modified oligonucleotide hybridization. This technique allows sensitive and highly specific detection of mature miRNAs.

PMID: 17889801 [PubMed - indexed for MEDLINE]