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Draft genome of the filarial nematode parasite Brugia malayi.
Ghedin E,
Wang S,
Spiro D,
Caler E,
Zhao Q,
Crabtree J,
Allen JE,
Delcher AL,
Guiliano DB,
Miranda-Saavedra D,
Angiuoli SV,
Creasy T,
Amedeo P,
Haas B,
El-Sayed NM,
Wortman JR,
Feldblyum T,
Tallon L,
Schatz M,
Shumway M,
Koo H,
Salzberg SL,
Schobel S,
Pertea M,
Pop M,
White O,
Barton GJ,
Carlow CK,
Crawford MJ,
Daub J,
Dimmic MW,
Estes CF,
Foster JM,
Ganatra M,
Gregory WF,
Johnson NM,
Jin J,
Komuniecki R,
Korf I,
Kumar S,
Laney S,
Li BW,
Li W,
Lindblom TH,
Lustigman S,
Ma D,
Maina CV,
Martin DM,
McCarter JP,
McReynolds L,
Mitreva M,
Nutman TB,
Parkinson J,
Peregrín-Alvarez JM,
Poole C,
Ren Q,
Saunders L,
Sluder AE,
Smith K,
Stanke M,
Unnasch TR,
Ware J,
Wei AD,
Weil G,
Williams DJ,
Zhang Y,
Williams SA,
Fraser-Liggett C,
Slatko B,
Blaxter ML,
Scott AL.
Division of Infectious Diseases, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA. GhedinE@dom.pitt.edu
Parasitic nematodes that cause elephantiasis and river blindness threaten hundreds of millions of people in the developing world. We have sequenced the approximately 90 megabase (Mb) genome of the human filarial parasite Brugia malayi and predict approximately 11,500 protein coding genes in 71 Mb of robustly assembled sequence. Comparative analysis with the free-living, model nematode Caenorhabditis elegans revealed that, despite these genes having maintained little conservation of local synteny during approximately 350 million years of evolution, they largely remain in linkage on chromosomal units. More than 100 conserved operons were identified. Analysis of the predicted proteome provides evidence for adaptations of B. malayi to niches in its human and vector hosts and insights into the molecular basis of a mutualistic relationship with its Wolbachia endosymbiont. These findings offer a foundation for rational drug design.
PMID: 17885136 [PubMed - indexed for MEDLINE]
PMCID: PMC2613796
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Cited by 23 PubMed Central articles
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Transcriptomic analysis of the entomopathogenic nematode Heterorhabditis bacteriophora TTO1.
Bai X, Adams BJ, Ciche TA, Clifton S, Gaugler R, Hogenhout SA, Spieth J, Sternberg PW, Wilson RK, Grewal PS.
BMC Genomics. 2009 Apr 30; 10:205. Epub 2009 Apr 30.
[BMC Genomics. 2009]
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Brugia malayi Excreted/Secreted Proteins at the Host/Parasite Interface: Stage- and Gender-Specific Proteomic Profiling.
Bennuru S, Semnani R, Meng Z, Ribeiro JM, Veenstra TD, Nutman TB.
PLoS Negl Trop Dis. 2009; 3(4):e410. Epub 2009 Apr 7.
[PLoS Negl Trop Dis. 2009]
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Evidence for metabolic provisioning by a common invertebrate endosymbiont, Wolbachia pipientis, during periods of nutritional stress.
Brownlie JC, Cass BN, Riegler M, Witsenburg JJ, Iturbe-Ormaetxe I, McGraw EA, O'Neill SL.
PLoS Pathog. 2009 Apr; 5(4):e1000368. Epub 2009 Apr 3.
[PLoS Pathog. 2009]
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