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Nan Fang Yi Ke Da Xue Xue Bao. 2007 Sep;27(9):1374-5.

[Dynamic analysis of HBV cccDNA in HepG2 cells infected with Ad-1.2 HBV].

[Article in Chinese]

Author information

  • 1Department of Infectious Diseases, Nanfang Hospital, Southern medical University, Guangzhou 510515, China.

Abstract

OBJECTIVE:

o study the replication of hepatitis B virus (HBV) in HepG2 cells infected with Ad-1.2 HBV.

METHODS:

HepG2 cells were transfected with adenovirus containing 1.2 copies of HBV DNA. The expression of HBV antigens were detected in the culture medium by means of enzyme-linked immunosorbent assay (ELISA), and the covalently closed circular DNA (cccDNA) in the cells was extracted with plasmid extraction kit and detected by real-time PCR with selective primer after treatment with mung bean nuclease.

RESULTS:

HBsAg, HBeAg and HBV cccDNA were all detected in HepG2 cells after tranfection with Ad-1.2 HBV. HBV cccDNA was detected 1 day after the infection, reaching the peak level 4 days after infection.

CONCLUSION:

Ad-1.2 HBV-infected cells can serve as the model for screening and evaluation of antiviral agents.

PMID:
17884782
[PubMed - indexed for MEDLINE]
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