Nat Methods. 2007 Oct;4(10):839-41. Epub 2007 Sep 16.

Speed-mapping quantitative trait loci using microarrays.

Lai CQ, Leips J, Zou W, Roberts JF, Wollenberg KR, Parnell LD, Zeng ZB, Ordovas JM, Mackay TF.

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Jean Mayer-US Department of Agriculture Human Nutrition Research Center on Aging, Nutrition and Genomics, Tufts University, 711 Washington Street, Boston, Massachusetts 02111, USA. chaoqiang.lai@ars.usda.gov

Abstract

We developed a rapid, economical method for high-resolution quantitative trait locus (QTL) mapping using microarrays for selective genotyping of pooled DNA samples. We generated 21,207 F2 flies from two inbred Drosophila melanogaster strains with known QTLs affecting lifespan, and hybridized DNA pools of young and old flies to microarrays. We used changes of gene frequency of 2,326 single-feature polymorphisms (SFPs) to map previously identified and additional QTLs affecting lifespan.

PMID:: 17873888; [PubMed - indexed for MEDLINE]

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