Bioorg Chem. 2007 Dec;35(6):458-64. Epub 2007 Sep 14.

PLP catabolism: identification of the 4-pyridoxic acid dehydrogenase gene in Mesorhizobium loti MAFF303099.

Source

Department of Chemistry and Chemical Biology, Cornell University, Ithaca, NY 14853, USA.

Abstract

The function of the mlr6793 gene from Mesorhizobium loti MAFF303099 has been identified. This gene encodes 4-pyridoxic acid dehydrogenase, an enzyme involved in the catabolism of PLP (Vitamin B6). This enzyme was overexpressed in Escherichia coli and characterized. 4-Pyridoxic acid dehydrogenase is a 33kDa protein that catalyzes the four electron oxidation of 4-pyridoxic acid to 3-hydroxy-2-methylpyridine-4,5-dicarboxylate, using nicotinamide adenine dinucleotide as a cofactor. The k cat for NADH production is 0.01s(-1). The KM values for 4-pyridoxic acid and NAD are 5.8 and 6.6microM, respectively.

PMID:: 17868768; [PubMed - indexed for MEDLINE]
PMCID:: PMC2140245

Free PMC Article

Images from this publication.See all images (2) Free text

Publication Types, MeSH Terms, Substances, Grant Support

Publication Types

Research Support, N.I.H., Extramural

MeSH Terms

Substances

Grant Support

LinkOut - more resources

Full Text Sources

Other Literature Sources

COS Scholar Universe

Molecular Biology Databases

PYRIDOXINE HYDROCHLORIDE - HSDB

Supplemental Content

Save items

Related citations in PubMed

Gene identification and characterization of 5-formyl-3-hydroxy-2-methylpyridine 4-carboxylic acid 5-dehydrogenase, an NAD+-dependent dismutase. [J Biochem. 2009]
Gene identification and characterization of 5-formyl-3-hydroxy-2-methylpyridine 4-carboxylic acid 5-dehydrogenase, an NAD+-dependent dismutase.
Yokochi N, Yoshikane Y, Matsumoto S, Fujisawa M, Ohnishi K, Yagi T. J Biochem. 2009 Apr; 145(4):493-503. Epub 2009 Jan 17.
Gene identification and characterization of the pyridoxine degradative enzyme 4-pyridoxic acid dehydrogenase from the nitrogen-fixing symbiotic bacterium Mesorhizobium loti MAFF303099. [J Biochem. 2008]
Gene identification and characterization of the pyridoxine degradative enzyme 4-pyridoxic acid dehydrogenase from the nitrogen-fixing symbiotic bacterium Mesorhizobium loti MAFF303099.
Ge F, Yokochi N, Yoshikane Y, Ohnishi K, Yagi T. J Biochem. 2008 May; 143(5):603-9. Epub 2008 Jan 23.
PLP catabolism: identification of the 2-(Acetamidomethylene)succinate hydrolase gene in Mesorhizobium loti MAFF303099. [Biochemistry. 2008]
PLP catabolism: identification of the 2-(Acetamidomethylene)succinate hydrolase gene in Mesorhizobium loti MAFF303099.
Mukherjee T, Hilmey DG, Begley TP. Biochemistry. 2008 Jun 10; 47(23):6233-41. Epub 2008 May 14.
Review Vitamin B6 metabolism by human liver. [Ann N Y Acad Sci. 1990]
Review Vitamin B6 metabolism by human liver.
Merrill AH Jr, Henderson JM. Ann N Y Acad Sci. 1990; 585:110-7.
Review Novel bioreduction system for the production of chiral alcohols. [Appl Microbiol Biotechnol. 2003]
Review Novel bioreduction system for the production of chiral alcohols.
Kataoka M, Kita K, Wada M, Yasohara Y, Hasegawa J, Shimizu S. Appl Microbiol Biotechnol. 2003 Oct; 62(5-6):437-45. Epub 2003 Jun 28.

See reviews... See all...

Cited by 2 PubMed Central articles

Structure determination and characterization of the vitamin B6 degradative enzyme (E)-2-(acetamidomethylene)succinate hydrolase. [Biochemistry. 2010]
Structure determination and characterization of the vitamin B6 degradative enzyme (E)-2-(acetamidomethylene)succinate hydrolase.
McCulloch KM, Mukherjee T, Begley TP, Ealick SE. Biochemistry. 2010 Feb 16; 49(6):1226-35.
Structure of the PLP degradative enzyme 2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase from Mesorhizobium loti MAFF303099 and its mechanistic implications. [Biochemistry. 2009]
Structure of the PLP degradative enzyme 2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase from Mesorhizobium loti MAFF303099 and its mechanistic implications.
McCulloch KM, Mukherjee T, Begley TP, Ealick SE. Biochemistry. 2009 May 19; 48(19):4139-49.

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Compound (MeSH Keyword)
PubChem chemical compound records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Gene
Gene records that cite the current articles. Citations in Gene are added manually by NCBI or imported from outside public resources.
Gene (GeneRIF)
Gene records that have the current articles as Reference into Function citations (GeneRIFs). NLM staff reviewing the literature while indexing MEDLINE add GeneRIFs manually.
Nucleotide (Weighted)
Nucleotide records associated with the current articles through the Gene database. These are the related sequences on the Gene record that are added manually by NCBI.
Protein (RefSeq)
NCBI protein Reference Sequences (RefSeqs) that are cited in the current articles, included in the corresponding Gene Reference into Function, or that include the PubMed articles as references.
Protein (Weighted)
Protein records associated with the current articles through related Gene database records. These are the related sequences on the Gene record that are added manually by NCBI.
Protein Clusters
Clusters of related proteins from the Protein Clusters database that cite the current articles. Sources of references in Protein Clusters include the associated Gene and Conserved Domain records as well as NCBI added citations.
References for this PMC Article
Citation referenced in PubMed article. Only valid for PubMed citations that are also in PMC.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Taxonomy via GenBank
Taxonomy records associated with the current articles through taxonomic information on related molecular database records (Nucleotide, Protein, Gene, SNP, Structure).
Free in PMC
Free full text articles in PMC
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

PLP catabolism: identification of the 4-pyridoxic acid dehydrogenase gene in Mes...
PLP catabolism: identification of the 4-pyridoxic acid dehydrogenase gene in Mesorhizobium loti MAFF303099.
Bioorg Chem. 2007 Dec ;35(6):458-64. Epub 2007 Sep 14 .

PubMed
A multimodal assessment of the relationship between emotion dysregulation and bo...
A multimodal assessment of the relationship between emotion dysregulation and borderline personality disorder among inner-city substance users in residential treatment.
J Psychiatr Res. 2008 Jul ;42(9):717-26. Epub 2007 Sep 14 .

PubMed
Occupational skin diseases caused by UV radiation.
Occupational skin diseases caused by UV radiation.
Acta Dermatovenerol Croat. 2007 ;15(3):191-8.

PubMed
[Importance of the radiographic sign of fecal loading in the cecum for the diffe...
[Importance of the radiographic sign of fecal loading in the cecum for the differential diagnosis of acute appendicitis].
Acta Med Port. 2007 Mar-Apr ;20(2):151-6. Epub 2007 Jun 11 .

PubMed
Efficacy, tolerability and comfort of a 0.3% hypromellose gel ophthalmic lubrica...
Efficacy, tolerability and comfort of a 0.3% hypromellose gel ophthalmic lubricant in the treatment of patients with moderate to severe dry eye syndrome.
Curr Med Res Opin. 2007 Nov ;23(11):2629-36.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to: