Three-dimensional nonlinear optical endoscopy

J Biomed Opt. 2007 Jul-Aug;12(4):040501. doi: 10.1117/1.2756102.

Abstract

The development of miniaturized nonlinear optical microscopy or endoscopy is essential to complement the current imaging modalities for diagnosis and monitoring of cancers. We report on a nonlinear optical endoscope based on a double-clad photonic crystal fiber and a two-dimensional (2-D) microelectromechanical system mirror, enabling the three-dimensional (3-D) nonlinear optical imaging through in vitro gastrointestinal tract tissue and human breast cancer tissue with a penetration depth of approximately 100 mum and axial resolution of 10 mum. The 3-D high-resolution and high-sensitive imaging ability of the nonlinear optical endoscope facilitates the visualization of 3-D morphologic and cell nuclei arrangement within tissue, and therefore will be important for histopathologic interpretation without the need of tissue excision.

Publication types

  • Evaluation Study
  • Letter
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Endoscopes, Gastrointestinal*
  • Equipment Design
  • Equipment Failure Analysis
  • Image Enhancement / instrumentation*
  • Image Enhancement / methods
  • Image Interpretation, Computer-Assisted / instrumentation*
  • Imaging, Three-Dimensional / instrumentation*
  • Imaging, Three-Dimensional / methods
  • Lenses*
  • Nonlinear Dynamics
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Tomography, Optical / instrumentation*
  • Tomography, Optical / methods