Abstract
Kaposi's sarcoma-associated herpesvirus (KSHV) is the etiological agent of Kaposi's sarcoma. The open reading frame (K9) of KSHV encodes viral interferon regulatory factor 1 (vIRF1), which functions as a repressor of interferon-mediated signal transduction. The amino-terminal region of vIRF1 displays significant homology to the DNA-binding domain of cellular interferon regulatory factors, supporting the theory that the protein interacts with specific DNA sequences. Here, we identify the consensus sequence of vIRF1-binding sites from a pool of random oligonucleotides. Moreover, our data show that vIRF1 interacts with the K3:viral dihydrofolate reductase:viral interleukin 6 promoter region in the KSHV genome.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Base Sequence
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Binding Sites
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Consensus Sequence
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DNA, Viral / chemistry
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DNA, Viral / metabolism*
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Electrophoretic Mobility Shift Assay
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Gene Expression Regulation, Viral*
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Herpesvirus 8, Human / genetics*
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Herpesvirus 8, Human / metabolism
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Humans
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Interferon Regulatory Factors / metabolism*
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Interleukin-6 / genetics
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Molecular Sequence Data
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Oligonucleotides / chemistry
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Oligonucleotides / metabolism
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Promoter Regions, Genetic
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Repressor Proteins / metabolism*
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Tetrahydrofolate Dehydrogenase / genetics
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Viral Proteins / genetics
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Viral Proteins / metabolism*
Substances
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DNA, Viral
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Interferon Regulatory Factors
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Interleukin-6
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K3 protein, Kaposi's sarcoma-associated herpesvirus
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Oligonucleotides
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Repressor Proteins
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Viral Proteins
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viral interferon regulatory factors
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Tetrahydrofolate Dehydrogenase