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J Clin Microbiol. 2007 Nov;45(11):3777-80. Epub 2007 Sep 5.

Comparison of plaque- and flow cytometry-based methods for measuring dengue virus neutralization.

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  • 1Department of Microbiology and Immunology, CB#7290, University of North Carolina School of Medicine, Chapel Hill, NC 27599, USA.

Abstract

As dengue vaccines enter clinical trials, there is a need for rapid and quantitative assays to measure neutralization. We have developed flow-based neutralization assays which generated results similar to those generated by the established, plaque reduction neutralization test. The flow assays are an improvement, as they use human cells and allow for high-throughput screening.

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