Abstract
Cell wall integrity signalling in Saccharomyces cerevisiae provides a model for the regulation of fungal wall biosynthesis. Chimers of the major plasma membrane sensors Wsc1 and Mid2 fused to GFP have been employed to show that intracellular and membrane distribution is only dependent on a membrane-anchored cytoplasmic tail. Phenotypic analyses of chimeric sensors in an isogenic Deltamid2 Deltawsc1 double deletion strain indicate that this tail, provided that it is linked to an extracellular domain, also determines the cellular response to different surface stresses to a large extent.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Calcium-Binding Proteins / chemistry
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Calcium-Binding Proteins / genetics
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Calcium-Binding Proteins / metabolism*
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Cell Wall / metabolism*
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Cytoplasm / metabolism
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Green Fluorescent Proteins / genetics
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Green Fluorescent Proteins / metabolism
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Intracellular Signaling Peptides and Proteins
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Membrane Glycoproteins
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Membrane Proteins / chemistry
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Membrane Proteins / genetics
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Membrane Proteins / metabolism*
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Microscopy, Fluorescence
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae / metabolism*
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Saccharomyces cerevisiae Proteins / chemistry
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Saccharomyces cerevisiae Proteins / genetics
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Saccharomyces cerevisiae Proteins / metabolism*
Substances
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Calcium-Binding Proteins
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Intracellular Signaling Peptides and Proteins
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MID2 protein, S cerevisiae
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Membrane Glycoproteins
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Membrane Proteins
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Recombinant Fusion Proteins
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SLG1 protein, S cerevisiae
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Saccharomyces cerevisiae Proteins
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Green Fluorescent Proteins