Mol Biochem Parasitol. 1991 Dec;49(2):239-43.

Genetic hybrids of Plasmodium falciparum identified by amplification of genomic DNA from single oocysts.

Ranford-Cartwright LC, Balfe P, Carter R, Walliker D.

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Institute of Cell, Animal and Population Biology, University of Edinburgh, Scotland, U.K.

Abstract

Individual oocysts from Plasmodium falciparum-infected Anopheles gambiae and Anopheles stephensi mosquitoes have been examined by the PCR technique, after their removal from the midgut. The DNA obtained from these oocysts has been amplified using oligonucleotide primers specific for part of the merozoite surface antigen MSA-1 gene. This technique distinguishes oocysts which are the products of self-fertilisation events from those which are the products of cross-fertilisation between different parasite clones.

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Genetic hybrids of Plasmodium falciparum identified by amplification of genomic ...
Genetic hybrids of Plasmodium falciparum identified by amplification of genomic DNA from single oocysts.
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Genetic hybrids of Plasmodium falciparum identified by amplification of genomic DNA from single oocysts.

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