Biol Chem Hoppe Seyler. 1991 Oct;372(10):923-7.

On the inhibition mechanism of the sialidase activity from Newcastle disease virus.

García Sastre A, Cobaleda C, Cabezas JA, Villar E.

Source

Departamento de Bioquímica y Biología Molecular, Facultad de Biología, Universidad de Salamanca, Spain.

Abstract

N-Acetylneuraminic, 2-deoxy-2,3-didehydro-N-acetylneuraminic acid and the beta anomer of methoxyneuraminic acid (Neu5Ac, Neu5Ac2en, MeONeu) have been used as probes for the catalytic mechanism of the activities of the outer membrane-bound haemagglutinin-neuraminidase (HN) from newcastle disease virus (NDV). Neu5Ac and Neu5Ac2en produced a competitive inhibition of the sialidase (= neuraminidase) activity, whereas MeONeu had no effect on this activity. This lack of inhibition can be explained by the free amino-acid group lacking the acetyl substituent in the MeONeu. Neu5Ac2en produced the highest inhibition. Based on the effect of the inhibitors, a reaction mechanism is suggested. On the other hand, the above mentioned inhibitors of the sialidase activity had no effect on haemagglutinating activity, suggesting different active sites for the both activities.

PMID:: 1772591; [PubMed - indexed for MEDLINE]

Publication Types, MeSH Terms, Substances

Publication Types

Research Support, Non-U.S. Gov't

MeSH Terms

Substances

LinkOut - more resources

Other Literature Sources

Labome Researcher Resource - ExactAntigen/Labome

Supplemental Content

Save items

Related citations in PubMed

Inhibition of neuraminidase activity by derivatives of 2-deoxy-2,3-dehydro-N-acetylneuraminic acid. [Virology. 1974]
Inhibition of neuraminidase activity by derivatives of 2-deoxy-2,3-dehydro-N-acetylneuraminic acid.
Meindl P, Bodo G, Palese P, Schulman J, Tuppy H. Virology. 1974 Apr; 58(2):457-63.
Inhibition of parainfluenza virus type 3 and Newcastle disease virus hemagglutinin-neuraminidase receptor binding: effect of receptor avidity and steric hindrance at the inhibitor binding sites. [J Virol. 2004]
Inhibition of parainfluenza virus type 3 and Newcastle disease virus hemagglutinin-neuraminidase receptor binding: effect of receptor avidity and steric hindrance at the inhibitor binding sites.
Porotto M, Murrell M, Greengard O, Lawrence MC, McKimm-Breschkin JL, Moscona A. J Virol. 2004 Dec; 78(24):13911-9.
Identification of amino acid residues important to the neuraminidase activity of the HN glycoprotein of Newcastle disease virus. [Virology. 1989]
Identification of amino acid residues important to the neuraminidase activity of the HN glycoprotein of Newcastle disease virus.
Iorio RM, Syddall RJ, Glickman RL, Riel AM, Sheehan JP, Bratt MA. Virology. 1989 Nov; 173(1):196-204.
Synthesis and evaluation of 4-O-alkylated 2-deoxy-2,3-didehydro-N-acetylneuraminic acid derivatives as inhibitors of human parainfluenza virus type-3 sialidase activity. [Bioorg Med Chem Lett. 2007]
Synthesis and evaluation of 4-O-alkylated 2-deoxy-2,3-didehydro-N-acetylneuraminic acid derivatives as inhibitors of human parainfluenza virus type-3 sialidase activity.
Tindal DJ, Dyason JC, Thomson RJ, Suzuki T, Ueyama H, Kuwahara Y, Maki N, Suzuki Y, von Itzstein M. Bioorg Med Chem Lett. 2007 Mar 15; 17(6):1655-8. Epub 2007 Jan 8.
Catalysis by a new sialidase, deaminoneuraminic acid residue-cleaving enzyme (KDNase Sm), initially forms a less stable alpha-anomer of 3-deoxy-D-glycero-D-galacto-nonulosonic acid and is strongly inhibited by the transition state analogue, 2-deoxy-2, 3-didehydro-D-glycero-D-galacto-2-nonulopyranosonic acid, but not by 2-deoxy-2,3-didehydro-N-acetylneuraminic acid. [J Biol Chem. 1997]
Catalysis by a new sialidase, deaminoneuraminic acid residue-cleaving enzyme (KDNase Sm), initially forms a less stable alpha-anomer of 3-deoxy-D-glycero-D-galacto-nonulosonic acid and is strongly inhibited by the transition state analogue, 2-deoxy-2, 3-didehydro-D-glycero-D-galacto-2-nonulopyranosonic acid, but not by 2-deoxy-2,3-didehydro-N-acetylneuraminic acid.
Terada T, Kitajima K, Inoue S, Wilson JC, Norton AK, Kong DC, Thomson RJ, von Itzstein M, Inoue Y. J Biol Chem. 1997 Feb 28; 272(9):5452-6.

See reviews... See all...

Cited by 1 PubMed Central article

Fusion between Newcastle disease virus and erythrocyte ghosts using octadecyl Rhodamine B fluorescence assay produces dequenching curves that fit the sum of two exponentials. [Biochem J. 1994]
Fusion between Newcastle disease virus and erythrocyte ghosts using octadecyl Rhodamine B fluorescence assay produces dequenching curves that fit the sum of two exponentials.
Cobaleda C, García-Sastre A, Villar E. Biochem J. 1994 Jun 1; 300 ( Pt 2):347-54.

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Compound (MeSH Keyword)
PubChem chemical compound records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

On the inhibition mechanism of the sialidase activity from Newcastle disease vir...
On the inhibition mechanism of the sialidase activity from Newcastle disease virus.
Biol Chem Hoppe Seyler. 1991 Oct ;372(10):923-7.

PubMed
Field-test of a date-rape drug detection device.
Field-test of a date-rape drug detection device.
J Anal Toxicol. 2007 Jul-Aug ;31(6):354-7.

PubMed
The temporal program of peripheral blood gene expression in the response of nonh...
The temporal program of peripheral blood gene expression in the response of nonhuman primates to Ebola hemorrhagic fever.
Genome Biol. 2007 ;8(8):R174.

PubMed
Interruption of agitation of platelet concentrates: a multicenter in vitro study...
Interruption of agitation of platelet concentrates: a multicenter in vitro study by the BEST Collaborative on the effects of shipping platelets.
Transfusion. 2007 Sep ;47(9):1666-73.

PubMed
Hippocampal long-term synaptic plasticity and signal amplification of NMDA recep...
Hippocampal long-term synaptic plasticity and signal amplification of NMDA receptors.
Crit Rev Neurobiol. 2006 ;18(1-2):71-84.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to: