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Genetics. 2007 Sep;177(1):615-29. Epub 2007 Aug 24.

The DrosDel deletion collection: a Drosophila genomewide chromosomal deficiency resource.

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  • 1Department of Genetics, University of Cambridge, Cambridge CB2 3EH, United Kingdom.

Abstract

We describe a second-generation deficiency kit for Drosophila melanogaster composed of molecularly mapped deletions on an isogenic background, covering approximately 77% of the Release 5.1 genome. Using a previously reported collection of FRT-bearing P-element insertions, we have generated 655 new deletions and verified a set of 209 deletion-bearing fly stocks. In addition to deletions, we demonstrate how the P elements may also be used to generate a set of custom inversions and duplications, particularly useful for balancing difficult regions of the genome carrying haplo-insufficient loci. We describe a simple computational resource that facilitates selection of appropriate elements for generating custom deletions. Finally, we provide a computational resource that facilitates selection of other mapped FRT-bearing elements that, when combined with the DrosDel collection, can theoretically generate over half a million precisely mapped deletions.

PMID:
17720900
[PubMed - indexed for MEDLINE]
PMCID:
PMC2013729
Free PMC Article

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