Zoo animals as reservoirs of gram-negative bacteria harboring integrons and antimicrobial resistance genes

Ashraf M Ahmed; Yusuke Motoi; Maiko Sato; Akito Maruyama; Hitoshi Watanabe; Yukio Fukumoto; Tadashi Shimamoto

doi:10.1128/AEM.01054-07

Zoo animals as reservoirs of gram-negative bacteria harboring integrons and antimicrobial resistance genes

Appl Environ Microbiol. 2007 Oct;73(20):6686-90. doi: 10.1128/AEM.01054-07. Epub 2007 Aug 24.

Authors

Ashraf M Ahmed¹, Yusuke Motoi, Maiko Sato, Akito Maruyama, Hitoshi Watanabe, Yukio Fukumoto, Tadashi Shimamoto

Affiliation

¹ Laboratory of Food Microbiology and Hygiene, Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima 739-8528, Japan. tadashis@hiroshima-u.ac.jp

Abstract

A total of 232 isolates of gram-negative bacteria were recovered from mammals, reptiles, and birds housed at Asa Zoological Park, Hiroshima prefecture, Japan. Forty-nine isolates (21.1%) showed multidrug resistance phenotypes and harbored at least one antimicrobial resistance gene. PCR and DNA sequencing identified class 1 and class 2 integrons and many beta-lactamase-encoding genes, in addition to a novel AmpC beta-lactamase gene, bla(CMY-26). Furthermore, the plasmid-mediated quinolone resistance genes qnr and aac(6')-Ib-cr were also identified.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Animals, Zoo / microbiology*
Anti-Bacterial Agents / pharmacology*
Drug Resistance, Bacterial / genetics*
Gram-Negative Bacteria / drug effects*
Gram-Negative Bacteria / genetics
Gram-Negative Bacteria / isolation & purification*
Integrons*
Microbial Sensitivity Tests
Molecular Sequence Data
Polymerase Chain Reaction / methods
Sequence Analysis, DNA
beta-Lactamases / genetics

Substances

Anti-Bacterial Agents
beta-Lactamases

Associated data

GENBANK/AB300358