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Proc Natl Acad Sci U S A. 2007 Aug 28;104(35):13936-41. Epub 2007 Aug 20.

High-throughput fluorescent-based optimization of eukaryotic membrane protein overexpression and purification in Saccharomyces cerevisiae.

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  • 1Division of Molecular Biosciences, Membrane Protein Crystallography Group, Imperial College, London SW7 2AZ, United Kingdom.

Abstract

Eukaryotic membrane proteins are often difficult to produce in large quantities, which is a significant obstacle for further structural and biochemical investigation. Based on the analysis of 43 eukaryotic membrane proteins, we present a cost-effective high-throughput approach for rapidly screening membrane proteins that can be overproduced to levels of >1 mg per liter in Saccharomyces cerevisiae. We find that 70% of the well expressed membrane proteins tested in this system are stable, targeted to the correct organelle, and monodisperse in either Fos-choline 12 (FC-12) or n-dodecyl-beta-D-maltoside. We illustrate the advantage of such an approach, with the purification of monodisperse human and yeast nucleotide-sugar transporters to unprecedented levels. We estimate that our approach should be able to provide milligram quantities for at least one-quarter of all membrane proteins from both yeast and higher eukaryotic organisms.

PMID:
17709746
[PubMed - indexed for MEDLINE]
PMCID:
PMC1955786
Free PMC Article

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