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Opt Lett. 2007 Aug 15;32(16):2348-50.

Microscope enabling multimodality imaging, angle-resolved scattering, and scattering spectroscopy.

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  • 1Institute of Optics and Departments of Physics and of Imaging Sciences, University of Rochester, Rochester, NY 14642, USA.

Abstract

We present the design, construction, and initial characterization of a multifunctional imaging/scattering spectroscopy system built around a commercial inverted microscope platform. The system enables co-registered brightfield, Fourier-filtered darkfield, and fluorescence imaging; monochromatic angle-resolved scattering measurements; and white-light wavelength-resolved scattering spectroscopy from the same field of view. A fiber-based illumination system provides illumination-wavelength flexibility and a good approximation to a point source. The performance of the system in its various data acquisition modes is experimentally verified using fluorescent microspheres. This multifunctional instrument provides a platform for studies on adherent cells from which the biophysical implications of subcellular light scattering can be studied in conjunction with sensitive fluorescence-based techniques.

PMID:
17700781
[PubMed - indexed for MEDLINE]
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