J Clin Microbiol. 2007 Oct;45(10):3443-5. Epub 2007 Aug 15.

Evaluation of pan-dermatophyte nested PCR in diagnosis of onychomycosis.

Garg J, Tilak R, Singh S, Gulati AK, Garg A, Prakash P, Nath G.

Source

Department of Microbiology, Banaras Hindu University, Varanasi, India. jaya_bhu@rediffmail.com

Abstract

In this study, nested PCR using novel primers targeting the pan-dermatophyte-specific sequence of the chitin synthase 1 gene (CHS1) was compared with KOH microscopy, culture isolation, and single-round PCR for diagnosis of 152 patients with clinically suspected onychomycosis. Results indicate that nested PCR may be considered the gold standard for the diagnosis of cases of onychomycosis for which the etiological agents are dermatophytes.

PMID:: 17699656; [PubMed - indexed for MEDLINE]
PMCID:: PMC2045349

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Research Support, Non-U.S. Gov't

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Chitin Synthase

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