Abstract
In mammals, mCRY proteins are essential and are major negative elements in circadian feedback loops. In this study, robust circadian clock oscillation was present even under conditions with constitutive over-expression of mCry1 in rat-1 cells. Rat-1 cells were produced to stably express mPer2 promoter-driven luciferase reporter, in which mCry1 was overexpressed under a tetracycline-dependent gene expression (Tet-On) system. Using these cells, we show that circadian clock oscillations in rat-1 fibroblasts persist when the mCRY1 protein constitutively accumulates in the nuclei.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Active Transport, Cell Nucleus / physiology
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Animals
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Biological Clocks / physiology*
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Cell Cycle Proteins / genetics
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Cell Cycle Proteins / metabolism
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Cell Line
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Cell Nucleus / genetics
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Cell Nucleus / metabolism*
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Circadian Rhythm / physiology*
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Cryptochromes
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Fibroblasts / metabolism*
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Flavoproteins / genetics
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Flavoproteins / metabolism*
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Gene Expression
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Genes, Reporter / physiology
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Luciferases / genetics
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Luciferases / metabolism
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Nuclear Proteins / genetics
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Nuclear Proteins / metabolism
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Period Circadian Proteins
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Rats
Substances
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Cell Cycle Proteins
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Cryptochromes
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Flavoproteins
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Nuclear Proteins
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Per2 protein, rat
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Period Circadian Proteins
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Luciferases