Mol Cell. 2007 Aug 3;27(3):509-16.

Structure of TOR and its complex with KOG1.

Adami A, García-Alvarez B, Arias-Palomo E, Barford D, Llorca O.

Source

Section of Structural Biology, Chester Beatty Laboratories, Institute of Cancer Research, 237 Fulham Road, London, UK.

Abstract

The target of rapamycin (TOR) is a large (281 kDa) conserved Ser/Thr protein kinase that functions as a central controller of cell growth. TOR assembles into two distinct multiprotein complexes: TORC1 and TORC2. A defining feature of TORC1 is the interaction of TOR with KOG1 (Raptor in mammals) and its sensitivity to a rapamycin-FKBP12 complex. Here, we have reconstructed in three dimensions the 25 A resolution structures of endogenous budding yeast TOR1 and a TOR-KOG1 complex, using electron microscopy. TOR features distinctive N-terminal HEAT repeats that form a curved tubular-shaped domain that associates with the C-terminal WD40 repeat domain of KOG1. The N terminus of KOG1 is in proximity to the TOR kinase domain, likely functioning to bring substrates into the vicinity of the catalytic region. A model is proposed for the molecular architecture of the TOR-KOG1 complex explaining its sensitivity to rapamycin.

PMID:: 17679098; [PubMed - indexed for MEDLINE]

Publication Types, MeSH Terms, Substances

Publication Types

Research Support, Non-U.S. Gov't

MeSH Terms

Substances

LinkOut - more resources

Full Text Sources

Molecular Biology Databases

Supplemental Content

Save items

Related citations in PubMed

Two TOR complexes, only one of which is rapamycin sensitive, have distinct roles in cell growth control. [Mol Cell. 2002]
Two TOR complexes, only one of which is rapamycin sensitive, have distinct roles in cell growth control.
Loewith R, Jacinto E, Wullschleger S, Lorberg A, Crespo JL, Bonenfant D, Oppliger W, Jenoe P, Hall MN. Mol Cell. 2002 Sep; 10(3):457-68.
Molecular organization of target of rapamycin complex 2. [J Biol Chem. 2005]
Molecular organization of target of rapamycin complex 2.
Wullschleger S, Loewith R, Oppliger W, Hall MN. J Biol Chem. 2005 Sep 2; 280(35):30697-704. Epub 2005 Jul 7.
Efficient Tor signaling requires a functional class C Vps protein complex in Saccharomyces cerevisiae. [Genetics. 2007]
Efficient Tor signaling requires a functional class C Vps protein complex in Saccharomyces cerevisiae.
Zurita-Martinez SA, Puria R, Pan X, Boeke JD, Cardenas ME. Genetics. 2007 Aug; 176(4):2139-50. Epub 2007 Jun 11.
Review Elucidating TOR signaling and rapamycin action: lessons from Saccharomyces cerevisiae. [Microbiol Mol Biol Rev. 2002]
Review Elucidating TOR signaling and rapamycin action: lessons from Saccharomyces cerevisiae.
Crespo JL, Hall MN. Microbiol Mol Biol Rev. 2002 Dec; 66(4):579-91, table of contents.
Review Identification of TOR signaling complexes: more TORC for the cell growth engine. [Cell. 2002]
Review Identification of TOR signaling complexes: more TORC for the cell growth engine.
Abraham RT. Cell. 2002 Oct 4; 111(1):9-12.

See reviews... See all...

Cited by 17 PubMed Central articles

LST8 regulates cell growth via target-of-rapamycin complex 2 (TORC2). [Mol Cell Biol. 2012]
LST8 regulates cell growth via target-of-rapamycin complex 2 (TORC2).
Wang T, Blumhagen R, Lao U, Kuo Y, Edgar BA. Mol Cell Biol. 2012 Jun; 32(12):2203-13. Epub 2012 Apr 9.
Mutations in the Arabidopsis homolog of LST8/GβL, a partner of the target of Rapamycin kinase, impair plant growth, flowering, and metabolic adaptation to long days. [Plant Cell. 2012]
Mutations in the Arabidopsis homolog of LST8/GβL, a partner of the target of Rapamycin kinase, impair plant growth, flowering, and metabolic adaptation to long days.
Moreau M, Azzopardi M, Clément G, Dobrenel T, Marchive C, Renne C, Martin-Magniette ML, Taconnat L, Renou JP, Robaglia C, et al. Plant Cell. 2012 Feb; 24(2):463-81. Epub 2012 Feb 3.
TORC2 signaling is antagonized by protein phosphatase 2A and the Far complex in Saccharomyces cerevisiae. [Genetics. 2012]
TORC2 signaling is antagonized by protein phosphatase 2A and the Far complex in Saccharomyces cerevisiae.
Pracheil T, Thornton J, Liu Z. Genetics. 2012 Apr; 190(4):1325-39. Epub 2012 Jan 31.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Compound (MeSH Keyword)
PubChem chemical compound records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Gene
Gene records that cite the current articles. Citations in Gene are added manually by NCBI or imported from outside public resources.
Gene (GeneRIF)
Gene records that have the current articles as Reference into Function citations (GeneRIFs). NLM staff reviewing the literature while indexing MEDLINE add GeneRIFs manually.
HomoloGene
HomoloGene clusters of homologous genes and sequences that cite the current articles. These are references on the Gene and sequence records in the HomoloGene entry.
Nucleotide (RefSeq)
NCBI nucleotide Reference Sequences (RefSeqs) that are cited in the current articles, included in the corresponding Gene Reference into Function, or that include the PubMed articles as references.
Nucleotide (Weighted)
Nucleotide records associated with the current articles through the Gene database. These are the related sequences on the Gene record that are added manually by NCBI.
Protein (RefSeq)
NCBI protein Reference Sequences (RefSeqs) that are cited in the current articles, included in the corresponding Gene Reference into Function, or that include the PubMed articles as references.
Protein (Weighted)
Protein records associated with the current articles through related Gene database records. These are the related sequences on the Gene record that are added manually by NCBI.
Protein Clusters
Clusters of related proteins from the Protein Clusters database that cite the current articles. Sources of references in Protein Clusters include the associated Gene and Conserved Domain records as well as NCBI added citations.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Taxonomy via GenBank
Taxonomy records associated with the current articles through taxonomic information on related molecular database records (Nucleotide, Protein, Gene, SNP, Structure).
Domains
Conserved Domain Database (CDD) records that cite the current articles. Citations are from the CDD source database records (PFAM, SMART).
GEO Profiles
Gene Expression Omnibus (GEO) Profiles of molecular abundance data. The current articles are references on the Gene record associated with the GEO profile.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Structure of TOR and its complex with KOG1.
Structure of TOR and its complex with KOG1.
Mol Cell. 2007 Aug 3 ;27(3):509-16.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to: