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Nucleic Acids Res. 2007;35(15):e97. Epub 2007 Aug 1.

Targeted high-throughput sequencing of tagged nucleic acid samples.

Author information

  • 1Max Planck Institute for Evolutionary Anthropology, Deutscher Platz 6, D-04103 Leipzig, Germany. mmeyer@eva.mpg.de

Abstract

High-throughput 454 DNA sequencing technology allows much faster and more cost-effective sequencing than traditional Sanger sequencing. However, the technology imposes inherent limitations on the number of samples that can be processed in parallel. Here we introduce parallel tagged sequencing (PTS), a simple, inexpensive and flexible barcoding technique that can be used for parallel sequencing any number and type of double-stranded nucleic acid samples. We demonstrate that PTS is particularly powerful for sequencing contiguous DNA fragments such as mtDNA genomes: in theory as many as 250 mammalian mtDNA genomes can be sequenced in a single GS FLX run. PTS dramatically increases the sequencing throughput of samples in parallel and thus fully mobilizes the resources of the 454 technology for targeted sequencing.

PMID:
17670798
[PubMed - indexed for MEDLINE]
PMCID:
PMC1976447
Free PMC Article

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