Polymorphism density across PDR5 between YJM789 and S288c. (A) Polymorphism distribution on chromosome XV from kilobases 600 to 640. Dashed lines indicate the start and stop positions of the PDR5 ORF. (B) The distribution of nonsynonymous and synonymous substitutions within the PDR5 ORF as determined from a 900-bp sliding window (each slide is 90 bp). The possibilities for nonsynonymous and synonymous substitutions were calculated as described previously (61). Red, nonsynonymous substitutions; green, synonymous substitutions; blue horizontal bars, transmembrane domains; vertical bars at the bottom, substitution sites.

Phylogenetic tree of YJM-GNAT homologs. (A) YJM-GNAT homologs were retrieved by BLASTP against the nonredundant database by using the threshold (E value of ≤1 × 10⁻⁵, identity of ≥30%, and the alignment matching at least 75% of the length of both query and subject sequences). Representative species are shown. Multiple alignments were built by CLUSTALW. A phylogenetic tree was then constructed by using the neighbor-joining method of the PHYLIP package. GNAT homologs are represented by their species names. (B and C) Phylogenies of two other YJM789 genes encoding acetyltrasferases for comparison: ELP3 (B) and ECM40 (C).

Alignment of the chromosome XIV sequences from YJM789 and S288c. (A) YJM789 contigs mapped to their locations on the S288c genome. (B) Sequence similarity between YJM789 contigs with a length of at least 10 kb and their corresponding sequences of S288c represented by color and coded from yellow (low) to orange (high). (C) Sequences of ≥100 bp that are present in YJM789 but absent in S288c are represented by blue lines. Similar sequences of <100 bp are represented by gray lines. (D) Sequence alignment between YJM789 and S288c chromosome XIV. Identical sequences are linked by lines. Red represents forward alignment. Green represents reverse complementary alignment. (E) Sequences of ≥100 bp that are absent in YJM789 but present in S288c are represented by blue lines. Similar sequences of <100 bp are represented by gray lines. (F) Repeat sequences of S288c are represented as follows: cyan rectangles, long terminal repeats; pink rectangles, retrotransposons; black rectangles, telomeres; black circle, centromere. (G) Coordinates of S288c in kilobase pairs.

Highly polymorphic region on chromosome I. (A) SNP distribution between YJM789 and S288c determined from a 1-kb sliding window over the nonrepeat sequence of S288c chromosome I. (B) Clustergram of the sequence similarity of chromosomes I of YJM789 compared with S288c, RM11-1a, and S. paradoxus (S. para) using a 1-kb sliding window. (C) Clustergram of the sequence similarity of the high polymorphism region of YJM789 chromosome I compared with S288c, RM11-1a, and S. paradoxus using a 100-bp sliding window. (D) Phylogeny of chromosome I sequences excluding the interval containing the high polymorphism region. The phylogenetic tree was constructed from nucleotide sequence alignments generated by using the program VISTA and the neighbor-joining method of the PHYLIP package. (E) Phylogeny of DUP240 region from YARWdelta6 to YARWdelta7 in S. paradoxus and all sequenced S. cerevisiae strains (38). A phylogenetic tree was constructed from nucleotide sequence alignments generated by CLUSTALW and the neighbor-joining method of the PHYLIP package. The scale bar indicates the evolutionary distance (number of substitutions per nucleotide position). (F) Alignments of YJM789, S288c, and S. paradoxus over the high polymorphism region using S288c (Upper) or YJM789 (Lower) as the reference sequence. The y axis represents the sequence similarity between two genomes along the reference sequence (graphs generated in VISTA). Sequence identity is shown for each pairwise comparison in a 100-bp sliding window. Note that differences in sequence lengths arise because of indels between YJM789 and S288c. Genes, as encoded in S288c, are represented by colored boxes: red, verified ORFs; pink, uncharacterized ORFs; gray, dubious ORFs; black, tRNAs and long terminal repeats.