Nucleic Acids Res. 1991 Dec 25;19(24):6819-21.

Requirement of protein factors and ATP for the disassembly of the spliceosome after mRNA splicing reaction.

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Department of Biophysics, Faculty of Science, Kyoto University, Japan.

Abstract

Pre-mRNA splicing reaction occurs in a large ribonucleoprotein complex called the spliceosome. After the splicing reaction, the spliceosome is disassembled to release the splicing products including spliced mRNA. Here we show that protein factors in a HeLa nuclear extract or a DEAE-cellulose fraction as well as ATP are required to release the splicing products form the spliceosome in which the splicing reaction has already completed.

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Pre-mRNA splicing within an assembled yeast spliceosome requires an RNA-dependent ATPase and ATP hydrolysis. [Proc Natl Acad Sci U S A. 1993]
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Requirement of protein factors and ATP for the disassembly of the spliceosome af...
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Requirement of protein factors and ATP for the disassembly of the spliceosome after mRNA splicing reaction.

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