J Mol Biol. 2007 Sep 7;372(1):1-6. Epub 2007 Jun 16.

Full-sequence computational design and solution structure of a thermostable protein variant.

Shah PS, Hom GK, Ross SA, Lassila JK, Crowhurst KA, Mayo SL.

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Biochemistry and Molecular Biophysics Option, MC 114-96, California Institute of Technology, Pasadena, CA 91125, USA.

Abstract

Computational protein design procedures were applied to the redesign of the entire sequence of a 51 amino acid residue protein, Drosophila melanogaster engrailed homeodomain. Various sequence optimization algorithms were compared and two resulting designed sequences were experimentally evaluated. The two sequences differ by 11 mutations and share 22% and 24% sequence identity with the wild-type protein. Both computationally designed proteins were considerably more stable than the naturally occurring protein, with midpoints of thermal denaturation greater than 99 degrees C. The solution structure was determined for one of the two sequences using multidimensional heteronuclear NMR spectroscopy, and the structure was found to closely match the original design template scaffold.

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Structures reported by this article

Full-Sequence Computational Design And Solution Structure Of A Thermostable Protein Variant

PDB: 2P6J

Source: unidentified

Method: Solution Nmr

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Full-sequence computational design and solution structure of a thermostable protein variant.

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