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Brain Res. 2007 Aug 8;1162:130-2. Epub 2007 Jun 19.

A novel duplex ELISA method for quantitation of plasma proteins in areas of brain edema.

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  • 1Neuroscience Graduate Program, University of Cincinnati College of Medicine, Cincinnati, OH, USA. loftspmc@email.uc.edu


We have developed a novel type of duplex enzyme-linked immunosorbent assay (ELISA) for the quantitation of the major plasma proteins, IgG and albumin, in edematous brain tissue. We test this duplex ELISA on our porcine intracerebral hemorrhage (ICH) model and show that it is as accurate and sensitive as independent single ELISAs. This method is useful as a marker of edema in brain tissue and the same design can be applied to other proteins and sample types.

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