Regulation of BRCA1 messenger RNA stability in human epithelial cell lines and during cell cycle progression

FEBS Lett. 2007 Jul 24;581(18):3435-42. doi: 10.1016/j.febslet.2007.06.046. Epub 2007 Jun 27.

Abstract

The mechanisms regulating expression of breast cancer 1 (BRCA1) are not fully characterised. By studying regulation of endogenous BRCA1 in human epithelial cell lines and during cell cycle progression, we provide evidence to suggest BRCA1 is regulated post-transcriptionally at the level of messenger RNA stability. We also show that RNA-binding proteins associate with an AU-rich, cis-active sequence of the BRCA1 3' untranslated region in a cell cycle-dependent manner. Our data identify a new post-transcriptional regulatory axis and a novel mechanism for modulating the levels of BRCA1 protein, with possible implications for understanding the mechanisms underlying BRCA1 repression in breast cancer.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • BRCA1 Protein / genetics
  • BRCA1 Protein / metabolism*
  • Base Sequence
  • Cell Cycle*
  • Cell Line
  • Epithelial Cells / cytology*
  • Epithelial Cells / metabolism*
  • Gene Expression Regulation
  • Humans
  • Protein Processing, Post-Translational
  • RNA Stability / genetics*
  • RNA, Messenger / genetics
  • Transcription, Genetic / genetics

Substances

  • BRCA1 Protein
  • RNA, Messenger