Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2007 Jun;32(3):437-42.

[Dicer efficiently converts large dsRNAs into siRNAs suitable for COX-2 gene].

[Article in Chinese]

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Department of Respiratory Diseases Second Xiangya Hospital, Central South University, Changsha 410011, China. luohong1003@163.com

Abstract

OBJECTIVE:

To report the use of Dicer to cleave double-stranded RNA (dsRNAs) into small interference RNAs (D-siRNAs) that can target multiple sites within an mRNA, and to acquire an new method to cure inflammation of the airway and tumor.

METHODS:

Using RiboMAX Large Scale RNA Production Systems-SP6 and T7 kit were used to transcribe A549 cell COX-2 DNA into RNA (dsRNAs). We mixed dsRNAs with Dicer in the reaction buffer. We recovered siRNAs using RNA Purification Column.

RESULTS:

Dicer efficiently converted double-stranded RNA of COX-2 into small interference RNAs of 21 approximately 23 bp.

CONCLUSION:

Dicer efficiently converts double-stranded RNA (dsRNA) into small interference RNAs (D-siRNAs of 21 approximately 23 bp).

PMID:: 17611321; [PubMed - indexed for MEDLINE]

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