Science. 2007 Aug 3;317(5838):632-8. Epub 2007 Jun 28.

Genome transplantation in bacteria: changing one species to another.

Lartigue C, Glass JI, Alperovich N, Pieper R, Parmar PP, Hutchison CA 3rd, Smith HO, Venter JC.

Source

J. Craig Venter Institute, Rockville, MD 20850, USA.

Abstract

As a step toward propagation of synthetic genomes, we completely replaced the genome of a bacterial cell with one from another species by transplanting a whole genome as naked DNA. Intact genomic DNA from Mycoplasma mycoides large colony (LC), virtually free of protein, was transplanted into Mycoplasma capricolum cells by polyethylene glycol-mediated transformation. Cells selected for tetracycline resistance, carried by the M. mycoides LC chromosome, contain the complete donor genome and are free of detectable recipient genomic sequences. These cells that result from genome transplantation are phenotypically identical to the M. mycoides LC donor strain as judged by several criteria.

PMID:: 17600181; [PubMed - indexed for MEDLINE]

LinkOut - more resources

Full Text Sources

Other Literature Sources

Supplemental Content

Save items

Related citations in PubMed

Creation of a bacterial cell controlled by a chemically synthesized genome. [Science. 2010]
Creation of a bacterial cell controlled by a chemically synthesized genome.
Gibson DG, Glass JI, Lartigue C, Noskov VN, Chuang RY, Algire MA, Benders GA, Montague MG, Ma L, Moodie MM, et al. Science. 2010 Jul 2; 329(5987):52-6. Epub 2010 May 20.
Transformation of Mycoplasma capricolum and examination of DNA restriction modification in M. capricolum and Mycoplasma mycoides subsp. mycoides. [Plasmid. 1994]
Transformation of Mycoplasma capricolum and examination of DNA restriction modification in M. capricolum and Mycoplasma mycoides subsp. mycoides.
King KW, Dybvig K. Plasmid. 1994 May; 31(3):308-11.
Comparison of the 16S-23S rRNA intergenic spacer regions among strains of the Mycoplasma mycoides cluster, and reassessment of the taxonomic position of Mycoplasma sp. bovine group 7. [Int J Syst Evol Microbiol. 2000]
Comparison of the 16S-23S rRNA intergenic spacer regions among strains of the Mycoplasma mycoides cluster, and reassessment of the taxonomic position of Mycoplasma sp. bovine group 7.
Harasawa R, Hotzel H, Sachse K. Int J Syst Evol Microbiol. 2000 May; 50 Pt 3:1325-9.
Review Transformation and DNA repair: linkage by DNA recombination. [Trends Microbiol. 2004]
Review Transformation and DNA repair: linkage by DNA recombination.
Tønjum T, Håvarstein LS, Koomey M, Seeberg E. Trends Microbiol. 2004 Jan; 12(1):1-4.
Review Mycoplasma pneumoniae and Mycoplasma genitalium: a comparison of two closely related bacterial species. [Curr Opin Microbiol. 1998]
Review Mycoplasma pneumoniae and Mycoplasma genitalium: a comparison of two closely related bacterial species.
Herrmann R, Reiner B. Curr Opin Microbiol. 1998 Oct; 1(5):572-9.

See reviews... See all...

Cited by 40 PubMed Central articles

The ten grand challenges of synthetic life. [Syst Synth Biol. 2011]
The ten grand challenges of synthetic life.
Porcar M, Danchin A, de Lorenzo V, Dos Santos VA, Krasnogor N, Rasmussen S, Moya A. Syst Synth Biol. 2011 Jun; 5(1-2):1-9. Epub 2011 Aug 5.
Enzymes are enriched in bacterial essential genes. [PLoS One. 2011]
Enzymes are enriched in bacterial essential genes.
Gao F, Zhang RR. PLoS One. 2011; 6(6):e21683. Epub 2011 Jun 28.
Mega-cloning and the advent of synthetic genomes. [Artif DNA PNA XNA. 2010]
Mega-cloning and the advent of synthetic genomes.
Goltermann L, Bentin T. Artif DNA PNA XNA. 2010 Jul; 1(1):54-57.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Compound (MeSH Keyword)
PubChem chemical compound records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Related Project
Related Projects
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Genome
Genome records that include the current articles as references. These are typically the articles that report the sequencing and analysis of the genome.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.
Cited in Books
NCBI Bookshelf books that cite the current articles.

Recent activity

Clear Turn Off Turn On

Genome transplantation in bacteria: changing one species to another.
Genome transplantation in bacteria: changing one species to another.
Science. 2007 Aug 3 ;317(5838):632-8. Epub 2007 Jun 28 .

PubMed
A one-session treatment for patients suffering from medically unexplained sympto...
A one-session treatment for patients suffering from medically unexplained symptoms in primary care: a randomized clinical trial.
Psychosomatics. 2007 Jul-Aug ;48(4):294-303.

PubMed
Revising the classification of somatoform disorders: key questions and prelimina...
Revising the classification of somatoform disorders: key questions and preliminary recommendations.
Psychosomatics. 2007 Jul-Aug ;48(4):277-85.

PubMed
Hypoxic switch in mitochondrial myeloid cell leukemia factor-1/Mtd apoptotic rhe...
Hypoxic switch in mitochondrial myeloid cell leukemia factor-1/Mtd apoptotic rheostat contributes to human trophoblast cell death in preeclampsia.
Am J Pathol. 2007 Aug ;171(2):496-506. Epub 2007 Jun 28 .

PubMed
Essential hypertension: a sign in search of a disease.
Essential hypertension: a sign in search of a disease.
CMAJ. 1991 Sep 1 ;145(5):384-5.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to:

Genome transplantation in bacteria: changing one species to another.

Source

Abstract

Publication Types, MeSH Terms, Substances

Publication Types

MeSH Terms

Substances

LinkOut - more resources

Full Text Sources

Other Literature Sources

Supplemental Content

Save items

Related citations in PubMed

Cited by 40 PubMed Central articles

Related information

Recent activity

Simple NCBI Directory

Getting Started

Resources

Popular

Featured

NCBI Information