Thermodynamic properties of the reactions in iAF1260. (A) The distribution of estimated Δ_rG′^m values for the reactions in iAF1260. Δ_rG′^m could be estimated for 1996 reactions (96%) in the reconstruction. 64% of the represented reactions have a negative Δ_rG′^m, and 20% of the reactions have a Δ_rG′^m of approximately zero. This distribution of Δ_rG′^m values indicates that most reactions in the model are thermodynamically favorable at millimolar concentration conditions. (B) The range of possible Δ_rG′ values for the reactions in iAF1260. Δ_rG′ differs from Δ_rG′^o (orange diamonds) and Δ_rG′^m (black diamonds) due to variations in metabolite concentrations from the 1 M and 1 mM reference states, respectively. Metabolite concentrations typically range from 0.02 to 0.00001 M, resulting in a wide range of values for Δ_rG′ (blue error bars). Taking uncertainty into account, the range of possible values for Δ_rG′ can be extended (purple error bars). The Δ_rG′ ranges were used to assess the feasibility and reversibility of the reactions in iAF1260; reactions for which a positive Δ_rG′ is not possible are thermodynamically irreversible.

Sensitivity analysis on the modeling parameters used in analyzing iAF1260. The relationship between the GUR (mmol gDW⁻¹ h⁻¹) (bottom axes, the dependant variable) and the resulting (1) GR (h⁻¹) (left axes) and (2) OUR (mmol gDW⁻¹ h⁻¹) (right axes) produced during the sensitivity analysis using iAF1260. Using FBA and iAF1260, optimal growth was simulated under glucose aerobic conditions while varying (A) the dry weight percentage of protein (50–80%), (B) RNA (10–25%) and (C) lipid (7–15%) in the BOF_CORE using physiologically measured values (Pramanik and Keasling, 1997). Also analyzed was (D) potential P/O ratios (1.0–2.7) in the network, as well as the (E) NGAM (±50%) and (F) GAM (±50%) that were determined for these conditions.

Classification of the ORFs, reactions and metabolites included in iAF1260. (A) Coverage of characterized ORFs from each of the COGs functional classes included in iAF1260 and five previous reconstructions. The percentage given is the total coverage accounted for in iAF1260 for each class. Some ORFs included in the reconstructions did not have a COG functional class assignment (see Supplemental information). (B) The number of reactions (both gene-associated and non-gene associated) that are associated to ORFs from each COG functional class. Since ORFs can belong to multiple classes, the percent unique in each class is listed. Non-gene-associated reactions were assigned to a class manually. (C) The number of metabolites that participate in reactions from each functional class and the percent unique in each class. Other (OT) includes classes J, K, L, O, T, U, V (see Supplementary information). NC, no COG assignment.

Utilizing iAF1260 as a predictive model. (A) A drawing of central metabolism and the ETS included in iAF1260. Originally, the entire network is unconstrained. (B) Application of transcriptional regulatory effects restricts the total number of pathways, or routes, flux can pass through in the network. (C) Further application of known reaction capacities can result in more accurate predictions. For example, the flux through the NADH dehydrogenase enzymes is split in a 1:1 ratio during a simulation to produce an optimal P/O ratio of approximately 1.4 (Gennis and Stewart, 1996; Noguchi et al, 2004). (D) The non-metabolic activity of the cell can be accounted for through maintenance parameters and these were approximated using experimental data under known media conditions. Chemostat data (see Materials and methods) was used (triangles) and the dotted line shows the modeling predictions with the appropriate maintenance parameters. (E) After the parameters are approximated, the model can then be used to predict the GR (circles), product formation (acetate, squares) and additional uptake rates (oxygen, triangles) under different environmental conditions (for succinate growth in this case).

ORF essentiality predictions using iAF1260. This heat map characterizes the agreement between ORFs predicted to be essential using iAF1260 and those experimentally determined from Baba et al (2006) and Joyce et al (2006). The enlarged region details how each a row corresponds to a computationally predicted essential ORF (188 total). The overall agreement between iAF1260 predictions and those found to be experimentally essential (overall, column 1) is shown along with a breakdown for ORFs found to be essential under rich media conditions (rich, column 2), under both glucose and glycerol minimal media conditions (shared, column 3) and under just glucose minimal medium conditions (glucose, column 4). ORFs are further grouped by their COG functional class (see Figure 1 for abbreviations; MU-ORF belongs to multiple COG classes). Dark blue indicates the condition under which each ORF was found to be essential. For example, folP was predicted to be an essential ORF for the biosynthesis of folate in iAF1260 under these conditions, but was not identified as essential by Baba et al (2006). This suggested the possibility of an alternative pathway for this step in E. coli that has yet to be characterized.