Format

Send to:

Choose Destination
See comment in PubMed Commons below
Eur Biophys J. 2007 Nov;36(8):1015-8. Epub 2007 Jun 20.

A new concept of olfactory biosensor based on interdigitated microelectrodes and immobilized yeasts expressing the human receptor OR17-40.

Author information

  • 1CEGELY, UMR-CNRS 5005, Ecole Centrale de Lyon, 36 Avenue Guy de Collongue, 69134, Ecully Cedex, France. mounamarrakchi@yahoo.fr

Abstract

This work shows the feasibility of an olfactory biosensor based on the immobilization of Saccharomyces cerevisiae yeast cells genetically modified to express the human olfactory receptor OR17-40 onto interdigitated microconductometric electrodes. This olfactory biosensor has been applied to the detection of its specific odorant (helional) with a high sensitivity (threshold 10(-14) M). In contrast, no significant response was observed using a non-specific odorant (heptanal), which suggests a good selectivity. Thus, this work may represent a first step towards a new kind of bioelectronic noses based on whole yeast cells and allowing a real time monitoring of olfactory receptor activation.

PMID:
17579849
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Springer
    Loading ...
    Write to the Help Desk