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1: FEBS Lett. 2007 Jul 10;581(17):3139-44. Epub 2007 Jun 6.Click here to read Links

ABCA3-mediated choline-phospholipids uptake into intracellular vesicles in A549 cells.

Matsumura Y, Sakai H, Sasaki M, Ban N, Inagaki N.

Department of Physiology, Akita University School of Medicine, Akita, Japan.

ABCA3 is proposed to function as a lung surfactant lipid transporter. Here we report ABCA3-dependent lipid uptake into intracellular vesicles in lung adenocarcinoma A549 cells. A549 cells stably expressing GFP-tagged wild-type ABCA3 (A549/ABCA3(WT)) had larger LAMP3-positive vesicles than their parental cells as well as A549 cells expressing a Walker A motif mutant (A549/ABCA3(N568D)). The choline-phospholipids level in A549/ABCA3(WT) was increased 1.25-fold compared to that in A549 and A549/ABCA3(N568D) cells, while the cholesterol levels were similar. Sucrose gradient fractionation analysis in A549/ABCA3(WT) cells revealed that choline-phospholipids were enriched in low-density and nile red-positive vesicles. Electronmicroscopic analysis showed multilamellar vesicles in A549/ABCA3(WT) cells. These results indicate that ABCA3 mediates ATP-dependent choline-phospholipids uptake into intracellular vesicles.

PMID: 17574245 [PubMed - indexed for MEDLINE]

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