Nat Methods. 2007 Jul;4(7):555-7. Epub 2007 Jun 17.

Bright monomeric red fluorescent protein with an extended fluorescence lifetime.

Merzlyak EM, Goedhart J, Shcherbo D, Bulina ME, Shcheglov AS, Fradkov AF, Gaintzeva A, Lukyanov KA, Lukyanov S, Gadella TW, Chudakov DM.

Source

Evrogen JSC, Miklukho-Maklaya 16/10, Moscow 117997, Russia.

Abstract

Fluorescent proteins have become extremely popular tools for in vivo imaging and especially for the study of localization, motility and interaction of proteins in living cells. Here we report TagRFP, a monomeric red fluorescent protein, which is characterized by high brightness, complete chromophore maturation, prolonged fluorescence lifetime and high pH-stability. These properties make TagRFP an excellent tag for protein localization studies and fluorescence resonance energy transfer (FRET) applications.

PMID:: 17572680; [PubMed - indexed for MEDLINE]

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