Titanium is the gold standard among materials used for prosthetic devices because of its good mechanical and chemical properties. There are three allotropic forms of titanium dioxide: brookite, rutile, and anatase. Anatase can be prepared as a colloidal suspension and used to coat surfaces. Anatase coating (AC) can potentially have biological effects and specifically can induce bone formation. To obtain more information about the osteogenic effect of AC in comparison to to titanium we used microRNA (miRNA) microarray techniques to investigate the translation regulation in osteoblasts exposed to both titanium and AC. There were three upregulated miRNAs (mir-1, mir-34c, mir-210) and eight downregulated miRNAs (mir-23b, mir-377, mir-22, mir-93, mir-422b, mir-17-5p, mir-24, mir-130b) for false discovery rate = 0 and score >3. The data reported are relevant to understand the molecular mechanism of bone regeneration and as a model for comparing other materials with similar clinical effects.