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LongSAGE profiling of nine human embryonic stem cell lines.
Hirst M,
Delaney A,
Rogers SA,
Schnerch A,
Persaud DR,
O'Connor MD,
Zeng T,
Moksa M,
Fichter K,
Mah D,
Go A,
Morin RD,
Baross A,
Zhao Y,
Khattra J,
Prabhu AL,
Pandoh P,
McDonald H,
Asano J,
Dhalla N,
Ma K,
Lee S,
Ally A,
Chahal N,
Menzies S,
Siddiqui A,
Holt R,
Jones S,
Gerhard DS,
Thomson JA,
Eaves CJ,
Marra MA.
Genome Sciences Centre, British Columbia Cancer Agency, Vancouver, British Columbia, Canada, V5Z 1L3.
To facilitate discovery of novel human embryonic stem cell (ESC) transcripts, we generated 2.5 million LongSAGE tags from 9 human ESC lines. Analysis of this data revealed that ESCs express proportionately more RNA binding proteins compared with terminally differentiated cells, and identified novel ESC transcripts, at least one of which may represent a marker of the pluripotent state.
PMID: 17570852 [PubMed - indexed for MEDLINE]
PMCID: PMC2394759
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Cited by 3 PubMed Central articles
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MicroRNA and gene expression patterns in the differentiation of human embryonic stem cells.
Ren J, Jin P, Wang E, Marincola FM, Stroncek DF.
J Transl Med. 2009 Mar 23; 7:20. Epub 2009 Mar 23.
[J Transl Med. 2009]
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Modeling transcriptome based on transcript-sampling data.
Zhu J, He F, Wang J, Yu J.
PLoS One. 2008 Feb 20; 3(2):e1659. Epub 2008 Feb 20.
[PLoS One. 2008]
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Application of massively parallel sequencing to microRNA profiling and discovery in human embryonic stem cells.
Morin RD, O'Connor MD, Griffith M, Kuchenbauer F, Delaney A, Prabhu AL, Zhao Y, McDonald H, Zeng T, Hirst M, et al.
Genome Res. 2008 Apr; 18(4):610-21. Epub 2008 Feb 19.
[Genome Res. 2008]