Acta Biochim Pol. 2007;54(2):235-44. Epub 2007 Jun 14.

Attaching a spin to a protein -- site-directed spin labeling in structural biology.

Czogalla A, Pieciul A, Jezierski A, Sikorski AF.

Source

Faculty of Biotechnology, University of Wrocław, Wrocław, Poland.

Abstract

Site-directed spin labeling and electron paramagnetic resonance spectroscopy have recently experienced an outburst of multiple applications in protein science. Numerous interesting strategies have been introduced for determining the structure of proteins and its conformational changes at the level of the backbone fold. Moreover, considerable technical development in the field makes the technique an attractive approach for the study of structure and dynamics of membrane proteins and large biological complexes at physiological conditions. This review focuses on a brief description of site-directed spin labeling-derived techniques in the context of their recent applications.

PMID:: 17565387; [PubMed - indexed for MEDLINE]

Free full text

Publication Types, MeSH Terms, Substances

Publication Types

MeSH Terms

Substances

LinkOut - more resources

Full Text Sources

Acta Biochemica Polonica, Inc. - PDF

Supplemental Content

Save items

Related citations in PubMed

Review Nitroxide spin-spin interactions: applications to protein structure and dynamics. [Annu Rev Biophys Biomol Struct...]
Review Nitroxide spin-spin interactions: applications to protein structure and dynamics.
Hustedt EJ, Beth AH. Annu Rev Biophys Biomol Struct. 1999; 28:129-53.
Combining high-field EPR with site-directed spin labeling reveals unique information on proteins in action. [Magn Reson Chem. 2005]
Combining high-field EPR with site-directed spin labeling reveals unique information on proteins in action.
Möbius K, Savitsky A, Wegener C, Plato M, Fuchs M, Schnegg A, Dubinskii AA, Grishin YA, Grigor'ev IA, Kühn M, et al. Magn Reson Chem. 2005 Nov; 43 Spec no.:S4-S19.
Review Identifying conformational changes with site-directed spin labeling. [Nat Struct Biol. 2000]
Review Identifying conformational changes with site-directed spin labeling.
Hubbell WL, Cafiso DS, Altenbach C. Nat Struct Biol. 2000 Sep; 7(9):735-9.
Review Recent advances and applications of site-directed spin labeling. [Curr Opin Struct Biol. 2006]
Review Recent advances and applications of site-directed spin labeling.
Fanucci GE, Cafiso DS. Curr Opin Struct Biol. 2006 Oct; 16(5):644-53. Epub 2006 Sep 1.
Methods for study of protein dynamics and protein-protein interaction in protein-ubiquitination by electron paramagnetic resonance spectroscopy. [Front Biosci. 2002]
Methods for study of protein dynamics and protein-protein interaction in protein-ubiquitination by electron paramagnetic resonance spectroscopy.
Steinhoff HJ. Front Biosci. 2002 Sep 1; 7:c97-110. Epub 2002 Sep 1.

See reviews... See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.

Recent activity

Clear Turn Off Turn On

Attaching a spin to a protein -- site-directed spin labeling in structural biolo...
Attaching a spin to a protein -- site-directed spin labeling in structural biology.
Acta Biochim Pol. 2007 ;54(2):235-44. Epub 2007 Jun 14 .

PubMed
Signal integration in the endoplasmic reticulum unfolded protein response.
Signal integration in the endoplasmic reticulum unfolded protein response.
Nat Rev Mol Cell Biol. 2007 Jul ;8(7):519-29.

PubMed
Topiramate-induced weight loss in schizophrenia: a retrospective case series stu...
Topiramate-induced weight loss in schizophrenia: a retrospective case series study.
Can J Clin Pharmacol. 2007 Summer ;14(2):e234-9. Epub 2007 Jun 12 .

PubMed
Fifty-year study of lung and bladder cancer mortality in Chile related to arseni...
Fifty-year study of lung and bladder cancer mortality in Chile related to arsenic in drinking water.
J Natl Cancer Inst. 2007 Jun 20 ;99(12):920-8. Epub 2007 Jun 12 .

PubMed
Prostate cancer progression and survival in BRCA2 mutation carriers.
Prostate cancer progression and survival in BRCA2 mutation carriers.
J Natl Cancer Inst. 2007 Jun 20 ;99(12):929-35. Epub 2007 Jun 12 .

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to: