See Figure 2 for details.

See Figure 2 for details.

Serine or threonine consensus sites are marked with their location (i.e. S12 is serine at residue 12). The key shows the font associated with each kinase's consensus site. Abbreviations used: MAPK, mitogen activated protein kinase; PKC, protein kinase C; CK2, casein kinase 2; GSK3, glycogen synthase kinase 3; PKA, protein kinase A. Consensus sites scanned using Scansite (http://scansite.mit.edu/) under moderate stringency. The sequence was scanned for the following kinase sites: Akt_Kin, ATM_Kin, Cam_Kin2, Casn_Kin1, Casn_Kin2, Cdc2_Kin, Cdk5_Kin, Clk2_Kin, DNA_PK, Erk1_Kin, GSK3_Kin, p38_Kin, PKA_Kin, PKC_common, PKC_delta, PKC_epsilon, PKC_mu, PKC_zeta.

Structure of PPARs. Panel A. Structure and functional domains of PPARα, β and γ. A/B is the hypervariable region containing the putative activation function-1 (AF-1) domain. PPARγ2 contains a 30 amino acid region that arises from differential promoter use and splicing. The C-domain is the most conserved and contains the DNA binding motif. The D-domain (hinge) is believed to allow for conformational change following ligand binding. The E/F domain contains the ligand-binding region of PPAR. Alignments and percent similarities were performed with MegAlign (DNAStar, Madison WI). Panel B. Detailed functional domains of PPARs. Above the outline for the hypothetical PPAR are the structural features of PPARs that have been deduced by sequence comparisons and crystallography. The AF-1 domain has not been fully characterized although it is known to reside in the A/B domain. The DNA binding motif contains two C₄ zinc fingers, the proximal (P-box) and distal (D-box) boxes, which confer DNA binding and heterodimerization, respectively. Much has been learned about PPAR structure/function from recent crystallographic studies. PPAR E/F contains 13 alpha helices (H1-H12, H2′) and 4 short β strands [60] and helices 3, 5 and 10 forms the ligand-binding pocket. RXR interacts along several helices including H7-H10. The coactivator CBP interacts with H3-5 and H12 while SRC-1 associated with H3, H5 and H12. The τ₁ domain contains a leucine-zipper-like heptad repeat [61]. The AF-2 domain is highly conserved among all PPARs and is intimately associated with ligand-induced transcriptional events. Much of the characterization of functional domains was performed using site-directed mutagenesis, as shown below the hypothetical PPAR. MAPK phosphorylation sites have been found at S12 and S21 in mouse PPARα and S122 in PPARγ2 [2]. Dominant negative (dn) PPARα results from mutations at L71, L123, and V444 [62] or in the naturally occurring truncated form of the receptor [63]. A dn PPARγ can be formed by mutating L468 and E471 of the human receptor [64]. Ligand binding mutants may arise from altering residues L319 or L469 of hPPARγ [65]. Similarly, DNA binding-devoid constructs are produced by mutating C122 [66] of PPARα. Specific interactions, such as those with SRC-1 and CBP, are targeted by mutating residues K301, V315, L468 or E471 of hPPARγ [65] while RXR association is lost by changing sites L433 [66], L370, L391 or D304 [61] of hPPARα. It is important to note that all domains work as a unified entity, with changes at the A/B terminus affecting ligand binding at the COOH, E/F domain [67] or in DNA binding [68]. Very little mutational analysis has been performed with PPARβ/γ, although the crystal structure reveals an E/F domain of this subtype to be very similar to PPARγ.