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Identification of clinical isolates of Leptospira spp by pulsed field gel-electrophoresis and microscopic agglutination test.

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  • 1Technical Office, Department of Medical Sciences, Ministry of Public Health, Tiwanon Road, Nonthaburi 11000, Thailand. pimjai@dmsc.moph.go.th

Abstract

Twenty-five clinical isolates of Leptospira spp were characterized by microscopic agglutination test (MAT) and pulsed field gel-electrophoresis (PFGE) in comparison with 23 reference Leptospira serovars and with the saprophytic L. biflexa serovar Patoc. PFGE DNA profiling was more specific and reliable than MAT.

PMID:
17539253
[PubMed - indexed for MEDLINE]
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