FRalanyzer results of the alignment of the conserved hypothetical protein EF3048 with the 3D-structure of pdaa protein (PDB code 1w1a). The alignment was obtained from the Bioinfo FR meta-server, (job ID 49416). For clarity, only the first 120 residues are shown (see our ‘samples’ url for the full alignment). The predicted secondary structures (H and E) of EF3048 are shown above its sequence. The experimental secondary structure of 1w1a is shown below its sequence. Identical residues are highlighted. PDBSum features of the template such as active site residues, contacts to metal and ligands are shown in separate rows and are marked as asterisks or hyphens. 1w1a belongs to the carbohydrate esterase family 4, and contains a conserved D and 3 H residues (D⁷³, H¹²⁴, H¹²⁸ and H²²²), which interact with the substrate. Other active site residues (e.g. G⁷⁵) annotated by PDBSum line the active site groove. The FRalanyzer results highlight the conservation of the residues D⁷³, G⁷⁵, H¹²⁴ and H²²². The alignment also shows that H¹²⁸ is shifted 2 residues from H⁹³ in the query, probably due to a misalignment in the FR result. Thus, by quickly visualizing the FR alignment, we are able to derive a verifiable hypothesis that identifies EF3048’s catalytic residues D¹², H⁶⁴, H⁹³ and H²¹⁵, suggesting that EF3048 is also a member of the carbohydrate esterase family 4.