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J Ethnopharmacol. 2007 Jul 25;112(3):590-4. Epub 2007 Apr 24.

Antibacterial and antifungal activity of Cassia fistula L.: an ethnomedicinal plant.

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  • 1Entomology Research Institute, Loyola College, Chennai 600034, India.


Hexane, chloroform, ethyl acetate, methanol and water extracts from the flower of Cassia fistula (an ethnomedicinal plant) were tested against bacteria and fungi. All the extracts exhibited antibacterial activity against Gram-positive organisms with minimum inhibitory concentrations (MIC) between 0.078 and 2.5 mg/ml. Among the Gram-negative bacteria, only Pseudomonas aeruginosa was susceptible to the extracts. Ethyl acetate crude extract was fractionated using chromatographic techniques. A crystal was isolated, which was confirmed as 4-hydroxy benzoic acid hydrate using X-ray crystallography. It exhibited antifungal activity against Trichophyton mentagrophytes (MIC 0.5 mg/ml) and Epidermophyton floccosum (MIC 0.5 mg/ml).

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