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J Chromatogr A. 2007 Jul 20;1157(1-2):422-9. Epub 2007 May 10.

Determination of conjugated linoleic acid in human plasma by fast gas chromatography.

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  • 1Department of Nutrition and Food Science, Reference Center in Food Technology, Faculty of Pharmacy, University of Barcelona, Avenida Joan XXIII s/n, E-08028 Barcelona, Spain.

Abstract

A new method for the determination of the main isomers of conjugated linoleic acid (CLA) in human and animal plasma was developed by gas chromatography coupled to flame ionization detection (GC-FID). The new method introduces three main advantages in comparison to the current available methodologies: firstly it does not require previous lipid extraction, secondly the chromatographic separation of CLA isomers was performed on an Rtx-2330 column significantly shorter and thinner than the typical long highly polar capillary columns in use that allows a faster analysis than in current methodologies, and thirdly the amount of sample needed to perform the analyses was substantially lower than the amount used in current routine methodologies. Its application to human plasma and rat plasma showed to be robust and reliable for quick and correct identification of the main CLA isomers in particular, and the total fatty acid profile in general, in routine analysis.

PMID:
17532324
[PubMed - indexed for MEDLINE]
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