Schematic of a fiber-based SD-OCT setup and a nerve chamber in its sample arm. The optical setup measures nerve movement relative to a stationary glass-saline interface. Nerve is positioned in a 20-mm-long and 1-mm-wide groove. Electrical stimulation and recording electrodes are made of platinum. Cross-sectional images can be acquired by scanning the beam over the sample laterally; however, the beam is stationary during the actual measurement. ASL, three element air-spaced lens; C, light collimator; G, transmission grating; LSC, line scan camera; SLD, superluminescent diode; Ti:Sapph, mode-locked titanium sapphire laser. Inset shows the optical read out.

Description of SD-OCT measurement to locate depth-resolved structures. (A) Optical spectrum at the detection arm of the interferometer, showing a strong modulation on the spectrum due to a single reflective surface in the sample arm. (B) Fourier transform of the optical spectrum, giving the spatial information in the sample arm. The peak at zero is a Fourier transform of the source spectrum; the peak at 240 μm is the reflective object causing modulations on the spectrum. The width of the peaks corresponds to axial resolution, which is inversely proportional to the bandwidth of the light source spectrum.

Optical detection of AP propagation in a crayfish leg nerve. (A) Depth profile showing the glass-saline interface and multiple surfaces within the nerve. Labels L₁ and L₂ indicate two locations inside the crayfish nerve. (B) Optical path length changes due to transient displacement during AP propagation at locations L₁ and L₂ and corresponding compound AP recorded differentially with respect to ground by a pair of platinum electrodes that are placed before and after the optical read-out area. Transient increase in optical signal represents a displacement toward the reference glass surface. Stimulus (1 mA, 50 μs) is presented at 2 ms and caused a localized artifact in the electrical measurement. Fifty responses are averaged in each trace.

Optical detection of AP propagation in a lobster leg nerve. (A) Cross-sectional SD-OCT image shows several nerve fibers. Horizontal line corresponds to the glass-saline interface. (B) Optical path length change due to transient displacement of the location indicated by the upper arrow during AP and corresponding compound AP recorded differentially with respect to ground by a pair of platinum electrodes that are placed before and after the optical read-out area. Optical signal represents a transient displacement toward the reference glass surface and suggests movement of the top surface of nerve fiber in swelling direction. Stimulus (300 μA, 50 μs) is presented at 2 ms and caused a localized artifact in the electrical measurement. (C) Optical path length change of a depth location indicated by the lower arrow represents a displacement during AP propagation that is away from the reference glass. (D) Optical path length increase measured between the two points indicated by the arrows shows that a reference glass may not be necessary for the measurement. One hundred responses are averaged in each trace.