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1: Genome Biol. 2007;8(5):R87.Click here to read Click here to read Links

Monitoring of gene knockouts: genome-wide profiling of conditionally essential genes.

Smith LK, Gomez MJ, Shatalin KY, Lee H, Neyfakh AA.

Center for Pharmaceutical Biotechnology, University of Illinois, Chicago, Illinois 60607, USA. lsmith28@uic.edu

We have developed a new microarray-based genetic technique, named MGK (Monitoring of Gene Knockouts), for genome-wide identification of conditionally essential genes. MGK identified bacterial genes that are critical for fitness in the absence of aromatic amino acids, and was further applied to identify genes whose inactivation causes bacterial cell death upon exposure to the bacteriostatic antibiotic chloramphenicol. Our findings suggest that MGK can serve as a robust tool in functional genomics studies.

PMID: 17519022 [PubMed - indexed for MEDLINE]

PMCID: PMC1929150