Aviat Space Environ Med. 2007 Apr;78(4 Suppl):A43-7.

16S rRNA beacons for bacterial monitoring during human space missions.

Larios-Sanz M, Kourentzi KD, Warmflash D, Jones J, Pierson DL, Willson RC, Fox GE.

Source

Department of Biology and Biochemistry, University of Houston, Houston, TX 77204, USA. maialarios@yahoo.com

Abstract

Microorganisms are unavoidable in space environments and their presence has, at times, been a source of problems. Concerns about disease during human space missions are particularly important considering the significant changes the immune system incurs during spaceflight and the history of microbial contamination aboard the Mir space station. Additionally, these contaminants may have adverse effects on instrumentation and life-support systems. A sensitive, highly specific system to detect, characterize, and monitor these microbial populations is essential. Herein we describe a monitoring approach that uses 16S rRNA targeted molecular beacons to successfully detect several specific bacterial groupings. This methodology will greatly simplify in-flight monitoring by minimizing sample handling and processing. We also address and provide solutions to target accessibility problems encountered in hybridizations that target 16S rRNA.

PMID:: 17511298; [PubMed - indexed for MEDLINE]

Publication Types, MeSH Terms, Substances

Publication Types

Research Support, U.S. Gov't, Non-P.H.S.

MeSH Terms

Substances

RNA, Ribosomal, 16S

LinkOut - more resources

Full Text Sources

Ingenta plc

Other Literature Sources

Supplemental Content

Save items

Related citations in PubMed

Microbial contamination of spacecraft. [Gravit Space Biol Bull. 2001]
Microbial contamination of spacecraft.
Pierson DL. Gravit Space Biol Bull. 2001 Jun; 14(2):1-6.
Review Microbial contamination of advanced life support (ALS) systems poses a moderate threat to the long-term stability of space-based bioregenerative systems. [Life Support Biosph Sci. 1998]
Review Microbial contamination of advanced life support (ALS) systems poses a moderate threat to the long-term stability of space-based bioregenerative systems.
Schuerger AC. Life Support Biosph Sci. 1998; 5(3):325-37.
Biological induced corrosion of materials II: new test methods and experiences from MIR station. [Acta Astronaut. 1999]
Biological induced corrosion of materials II: new test methods and experiences from MIR station.
Klintworth R, Reher HJ, Viktorov AN, Bohle D. Acta Astronaut. 1999 Apr-Jun; 44(7-12):569-78.
Identification and characterization of bacterial isolates from the Mir space station. [Microbiol Res. 2005]
Identification and characterization of bacterial isolates from the Mir space station.
Song B, Leff LG. Microbiol Res. 2005; 160(2):111-7.
Review Adsorption processes in spacecraft environmental control and life support systems. [Stud Surf Sci Catal. 1999]
Review Adsorption processes in spacecraft environmental control and life support systems.
DallBauman LA, Finn JE. Stud Surf Sci Catal. 1999; 120:455-71.

See reviews... See all...

Cited by 1 PubMed Central article

Review Space microbiology. [Microbiol Mol Biol Rev. 2010]
Review Space microbiology.
Horneck G, Klaus DM, Mancinelli RL. Microbiol Mol Biol Rev. 2010 Mar; 74(1):121-56.

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

16S rRNA beacons for bacterial monitoring during human space missions.
16S rRNA beacons for bacterial monitoring during human space missions.
Aviat Space Environ Med. 2007 Apr ;78(4 Suppl):A43-7.

PubMed
[Clarification of the pathogenesis and development of clinical examination for a...
[Clarification of the pathogenesis and development of clinical examination for allergic diseases].
Rinsho Byori. 2007 Apr ;55(4):369-74.

PubMed
The worldwide prevalence of ADHD: is it an American condition?
The worldwide prevalence of ADHD: is it an American condition?
World Psychiatry. 2003 Jun ;2(2):104-13.

PubMed
The evolving epidemiology of bipolar disorder.
The evolving epidemiology of bipolar disorder.
World Psychiatry. 2002 Oct ;1(3):146-8.

PubMed
Randomized controlled trial of specific spinal stabilization exercises and conve...
Randomized controlled trial of specific spinal stabilization exercises and conventional physiotherapy for recurrent low back pain.
Spine (Phila Pa 1976). 2006 Sep 1 ;31(19):E670-81.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to: