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Biophys J. 2007 Aug 1;93(3):1032-8. Epub 2007 May 4.

Light-induced dielectrophoretic manipulation of DNA.

Author information

  • 1Walter Schottky Institut, Technische Universität München, Garching, Germany. hoeb@wsi.tum.de

Abstract

Light-induced dielectrophoretic movement of polystyrene beads and lambda-DNA is studied using thin films of amorphous hydrogenated silicon as local photoaddressable electrodes with a diameter of 4 microm. Positive (high-field seeking) dielectrophoretic movement is observed for both types of objects. The absence of strong negative (low-field seeking) dielectrophoresis of DNA at high frequencies is in agreement with the similarity of the dielectric constants of DNA and water, the real part of the dielectric function. The corresponding imaginary part of the dielectric function governed by the conductivity of DNA can be determined from a comparison of the frequency dependence of the dielectrophoretic drift velocity with the Clausius-Mossotti relation.

PMID:
17483160
[PubMed - indexed for MEDLINE]
PMCID:
PMC1913157
Free PMC Article
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