Abstract

To confirm the modulation role of GABA(B) on ethanol' effects, we studied the effects of ethanol on the neuronal intracellular signals, protein kinase A (PKA) and cAMP-response element binding protein (CREB), by using a system where GABA(B1) receptors were specifically knocked down in the in vitro cultivated cortical neurons. The results showed that the PKA alpha subunit was increased with ethanol treatment, and could be further increased by administering baclofen and phaclofen. By contrast, baclofen and/or phaclofen could decrease ethanol's up-regulation effects on PKA alpha subunit expression in primary cultured cortical neurons in which the GABA(B1) receptor was specifically knocked down using GABA(B1) receptor RNA interference. Furthermore, these effects could lead to changes of phospho (p)-CREB expression, which showed the same expression pattern as PKA. Finally, we observed changes of GABA(B1), PKA, and p-CREB distribution within the same neuronal cells. These results showed that the GABA(B) receptors are critical to ethanol's cellular effects, which occur via modulating the PKA and CREB transcription pathway, and may be an underlying cause of ethanol's effects.