Format

Send to:

Choose Destination
See comment in PubMed Commons below
Appl Environ Microbiol. 2007 Jun;73(12):3896-908. Epub 2007 Apr 27.

Stable isotope ratios and forensic analysis of microorganisms.

Author information

  • 1Pacific Northwest National Laboratory, 999 Battelle Blvd., P8-13, Richland, WA 99352, USA. Helen.kreuzer@pnl.gov

Abstract

In the aftermath of the anthrax letters of 2001, researchers have been exploring various analytical signatures for the purpose of characterizing the production environment of microorganisms. One such signature is stable isotope ratios, which in heterotrophs, are a function of nutrient and water sources. Here we discuss the use of stable isotope ratios in microbial forensics, using as a database the carbon, nitrogen, oxygen, and hydrogen stable isotope ratios of 247 separate cultures of Bacillus subtilis 6051 spores produced on a total of 32 different culture media. In the context of using stable isotope ratios as a signature for sample matching, we present an analysis of variations between individual samples, between cultures produced in tandem, and between cultures produced in the same medium but at different times. Additionally, we correlate the stable isotope ratios of carbon, nitrogen, oxygen, and hydrogen for growth medium nutrients or water with those of spores and show examples of how these relationships can be used to exclude nutrient or water samples as possible growth substrates for specific cultures.

PMID:
17468274
[PubMed - indexed for MEDLINE]
PMCID:
PMC1932740
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for HighWire Icon for PubMed Central
    Loading ...
    Write to the Help Desk